Article

Abdominocentesis in cattle: technique and criteria for diagnosis of peritonitis.

The Canadian veterinary journal. La revue veterinaire canadienne (Impact Factor: 0.47). 02/1985; 26(2):74-80.
Source: PubMed

ABSTRACT A reliable method for the collection of peritoneal fluid from cattle using a trocar and cannula is described. Peritoneal fluid was collected from three groups of cattle: periparturient, normal and with peritonitis. The fluid was examined by white cell count, differential cell count, total protein concentration and bacteriology. The results were analysed to determine the best criteria for peritonitis. Greater than 10% eosinophils were typical of normal peritoneal fluid. Peritoneal fluid with a relative neutrophil count greater than 40% and a relative eosinophil count of less than 10% was frequently associated with the diagnosis of peritonitis. Parturient cattle had large volumes of peritoneal fluid with low total protein and white cell counts. Growth of Gram-negative or anaerobic organisms was associated with mortality.

1 Follower
 · 
672 Views
  • Source
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of this study was: (1) to establish a method for percutaneous ultrasound-guided aspiration of peritoneal fluid (PF) in healthy camels, (2) to assess the PF constituents, and (3) to compare these concentrations with those in serum of the same animals. From 15 healthy camels (Camelus dromedarius), whole blood, serum samples, as well as PF, were collected and serum biochemistry was done to compare PF values. All serum and PF samples was examined for comp. By means of ultrasonography, free PF was imaged in two locations; anteriorly in the triangular space between the dorsal ruminal sac and reticulum and; posteriorly 10-cm cranial to the umbilicus. In 12 of the 15 camels, the fluid was visualised in the 2 locations. Free PF was only visible anteriorly in 2 animals. We could not image free PF in the other location. The volume of PF ranged from 3 to 18 mL (mean 11 ± 4.7 mL); it was clear and colourless. Few white blood cells were found in the PF samples (0.10 ± 0.03 × 109 cells/L). Compared to serum values, total protein, albumin and globulin were lower in PF (p < 0.001). Similarly, the serum activity of aspartate aminotransferase, alkaline phosphatase and creatine kinase were lower in PF compared to serum values (p < 0.05). This study gives promising results concerning the normal composition of PF and will be useful in the assessment of PF in diseased camels.
    Small Ruminant Research 06/2013; 113(1):307–311. DOI:10.1016/j.smallrumres.2013.04.002 · 1.10 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The goals of this study were to evaluate techniques for collection of peritoneal fluid from calves, establish reference ranges for fibrinogen in peritoneal fluid during the 1st month of life, and determine if abomasal puncture would alter peritoneal fluid or hematologic variables. Twenty-two healthy Holstein calves underwent 3 peritoneal fluid collections on day 1, day 15, and day 30 of age. Fibrinogen concentration in peritoneal fluid was 0.20 g/dL and 0.10 g/dL (P < .05) for day 1 and day 30, respectively, and 0.10 at day 15 (P > .05) for calves without abomasal puncture. Plasma fibrinogen concentration was 0.60 g/dL and 0.70 g/dL (P < .05) for days 15 and 30, respectively, in calves without abomasal puncture. There were no significant differences (P≤ .05) in peritoneal fluid and peripheral blood total protein and fibrinogen concentrations, specific gravity, total and differential cell count, or erythrocyte counts between calves with or without abomasal puncture. We concluded that the reference ranges established for fibrinogen and total protein concentration are important for accurate evaluation of peritoneal fluid in calves for further comparison with similar-aged animals with gastrointestinal-tract or abdominal-cavity disease. Additionally, accidental abomasal puncture does not alter values of fibrinogen, total protein, and nucleated cell count in peritoneal fluid and does not cause apparent clinical abnormalities.
    Journal of Veterinary Internal Medicine 06/2008; 19(6):899 - 904. DOI:10.1111/j.1939-1676.2005.tb02785.x · 2.22 Impact Factor

Preview

Download
12 Downloads
Available from