Composition and antifungal activity of essential oils of Mentha piperita and Lavendula angustifolia on post-harvest phytopathogens.
ABSTRACT The general antifungal activity of essential oils is well documented. The advantage of essential oils is their bioactivity in the vapor phase, a characteristic that makes them attractive as possible fumigants for stored product protection. Essential oils of aerial parts of Mentha piperita and Lavendula angustifolia were obtained with hydrodistillation and oils composition identified with GC-MS. Menthanol (36.24%) and menthone (32.42%) were the major compounds of the M. piperata essential oil. The essential oil of L. angustifolia was rich in linalool (49.2%) , linalyl acetate (12.3%), Lavendul acetate (6.5%), 4-terpineol (5.9%). Fungal toxicity of the essential oils were evaluated against three pathogenic fungi (Rhizopus stolonifer, Botrytis cinerea and Aspergillus niger) in vitro. Plate assayes showed that the different concentrations of essential oils have antifungal activity against these fungi, and the essential oil of L. angustifolia showed stronger fungistatic activity. Lavendula oil exhibited complete growth inhibition of all pathogens at 1000 ppm and minimum EC50 (311.24 ppm) resulted on B. cinerea.
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ABSTRACT: Oriental herbs are reported as having potent functions for preventing many types of diseases. They also appear to have positive effects and potential capabilities for skin care. Among the many oriental herbs that are available, we chose to analyze four medicinal herbs, Korean red ginseng, Artemisia capillaries Thunb, Schizonepeta tenuifolia Briq, and Foeniculum vulgare Mill, because all are popular and considered as favorite medicinal plants in Korea. Extracts of the herbs were obtained by various methods such as using distilled water, ethyl ether, methanol, ethanol, benzene, 1-butanol, and chloroform. Nine phytochemicals were detected in the extracts: maltol, adenosine, b-pinene, menthone, pulegone, limonene, anethole, estragole, and fenchone, which reportedly have multi-functionalities. All phytochemicals were analyzed quantitatively by various chromatographic techniques such as HPLC and gas chromatography-mass (GC-MS) spectrometry. This article also presents the optimum conditions for extracting these 9 targeted phytochemical compounds that were derived from 4 popular oriental herbs, which could be useful for the efficient preparation of each phytochemical.01/2008; 40(3).
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ABSTRACT: S u m m a r y The aim of this study was the estimation of influence of essential oils deriving from two varieties of peppermint: white mint (Mentha x piperita L var. officinalis Sole f. palles-cens Camus) and bergamot mint (Mentha x piperita L. var. citrata Ehrh.), on the growth of some filamentous fungi: Aspergillus niger, Botrytis cinerea, Eurotium amstelodami, Eu-rotium chevalieri, Penicillium cyclopium and Trichothecium roseum. The oils were extracted from dried, ground herb by means of hydro-distillation method. The disc diffusion method was used to estimation an antifungal activity of oils (doses of oil: 10 and 5 µl/disc). The diameters of growth inhibition zones were measured and expressed in mm. It was stated that the oils from both varieties of peppermint inhibited the growth of all tested fungi. The activity of oils was significantly differentiated in dependence on kind of oil, dose of oil and species of fungi. The oil from bergamot mint (in both doses) was more effective to most of the tested strains than the oil from white mint. It also possessed a greater content of carvone and pulegone. Usually, the inhibition growth zones at the oil dose of 10 µl/disc were proportionally bigger than at dose of 5 µl/disc. The strains of P. cyclopium and A. niger were most resistant to the influence of both oils – the average inhibition growth zones were 12.4 and 16.7 mm, respectively. E. chevalieri and E. amstelodami were most sensitive – average zones of their growth inhibition amounted to 90.0 and 75.9 mm, respectively.