Composition and antifungal activity of essential oils of Mentha piperita and Lavendula angustifolia on post-harvest phytopathogens.
ABSTRACT The general antifungal activity of essential oils is well documented. The advantage of essential oils is their bioactivity in the vapor phase, a characteristic that makes them attractive as possible fumigants for stored product protection. Essential oils of aerial parts of Mentha piperita and Lavendula angustifolia were obtained with hydrodistillation and oils composition identified with GC-MS. Menthanol (36.24%) and menthone (32.42%) were the major compounds of the M. piperata essential oil. The essential oil of L. angustifolia was rich in linalool (49.2%) , linalyl acetate (12.3%), Lavendul acetate (6.5%), 4-terpineol (5.9%). Fungal toxicity of the essential oils were evaluated against three pathogenic fungi (Rhizopus stolonifer, Botrytis cinerea and Aspergillus niger) in vitro. Plate assayes showed that the different concentrations of essential oils have antifungal activity against these fungi, and the essential oil of L. angustifolia showed stronger fungistatic activity. Lavendula oil exhibited complete growth inhibition of all pathogens at 1000 ppm and minimum EC50 (311.24 ppm) resulted on B. cinerea.
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ABSTRACT: Variations in quantity and quality of essential oil (EO) from the aerial parts of cultivated Mentha piperita were determined. The EO of air-dried sample was obtained by a hydrodistillation method and analyzed by a gas chromatography/mass spectrometry (GC/MS). The antifungal activity of the EO was investigated by broth microdilution methods as recommended by Clinical and Laboratory Standards Institute. A biofilm formation inhibition was measured by using an XTT reduction assay. Menthol (53.28%) was the major compound of the EO followed by Menthyl acetate (15.1%) and Menthofuran (11.18%). The EO exhibited strong antifungal activities against the examined fungi at concentrations ranging from 0.12 to 8.0 μL/mL. In addition, the EO inhibited the biofilm formation of Candida albicans and C. dubliniensis at concentrations up to 2 μL/mL. Considering the wide range of the antifungal activities of the examined EO, it might be potentially used in the management of fungal infections or in the extension of the shelf life of food products.ISRN pharmaceutics. 01/2012; 2012:718645.
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ABSTRACT: The antioxidant activities and the determined major components of six popular and commercially available herb essential oils, including lavender (Lavendular angustifolia), peppermint (Mentha piperita), rosemary (Rosmarius officinalis), lemon (Citrus limon), grapefruit (Citrus paradise), and frankincense (Boswellia carteri), were compared. The essential oils were analysed by GC-MS and their antioxidant activities were determined by testing free radical-scavenging capacity and lipid peroxidation in the linoleic acid system. The major components of the essential oils of lavender, peppermint, rosemary, lemon, grapefruit, and frankincense were linalyl acetate (28.2%), menthol (33.4%), 1,8-cineole (46.1%), limonene (64.5 and 94.2%), and p-menth-2-en-ol (34.5%), respectively. The highest DPPH radical-scavenging activity was obtained by the lavender essential oil and limonene, with RC50 values of 2.1 +/- 0.23% and 2.1 +/- 0.04%, respectively. Radical-scavenging activity against the ABTS radical was highest in peppermint essential oil (1.6 +/- 0.09). Lavender oil was most effective for inhibiting linoleic acid peroxidation after 10 days.Natural product research 01/2010; 24(2):140-51. · 1.01 Impact Factor
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ABSTRACT: The sweet herb of Paraguay, Stevia rebaudiana (Bertoni), is becoming more important worldwide in herbal care for diabetes, as it produces the zero-calorie sweeteners steviol glycosides (SGs)—stevioside and rebaudioside-A. While arbuscular mycorrhizal fungi (AMF) have been shown to enhance production of secondary metabolites in many plant species, their effect on S. rebaudiana has not been studied. Moreover, relatively little is known about the mechanisms that may be involved in the increased accumulation of phytochemicals in mycorrhizal plants. Therefore, this study was performed to test the ability of Rhizophagus fasciculatus (Thaxt.) C. Walker & A. Schüßler to improve the yield of SGs in S. rebaudiana and to relate this with some AMF-induced physiological changes in addition to improved phosphorus (P) uptake. The performance of plants inoculated with R. fasciculatus was compared with that of non-mycorrhizal plants with similar P concentrations. Mycorrhizal (M) and non-mycorrhizal plants with P-supplementation (NM + P) produced higher concentrations of SGs compared with control plants. However, M plants had more SGs than did NM + P plants. The higher content of SGs in M plants is due to increased concentrations of SGs and to the enhanced biomass of the shoots. The increase in biomass is directly due to the improved uptake of nutrients (N, K, Mg, Cu, Fe, Mn and Zn), and chlorophyll and carbohydrate concentrations in M plants. Higher concentrations of total carbohydrates and jasmonic acid in M plants than in NM + P plants contribute to more biosynthesis of SGs via the methyl erythritol phosphate pathway. This study suggests that AMF-mediated increases in SGs involve both nutritionally and non-nutritionally linked mechanismsApplied Soil Ecology 01/2013; 72:187-194. · 2.11 Impact Factor