Article

Interstitial transfer factor as adjuvant immunotherapy for experimental glioma

Neuroimmunology Unit, National Institute of Neurology and Neurosurgery of Mexico, Mexico City.
Journal of experimental & clinical cancer research: CR (Impact Factor: 3.27). 12/2005; 24(4):575-83.
Source: PubMed

ABSTRACT Glioblastoma multiform (GBM) is the most common tumour of the central nervous system in humans. Unfortunately its prognosis is poor and because of the lack of efficacious therapies, immunotherapy is a potential treatment. Transfer factors (TF) are low molecular weight dialysable products extracted from immune cells which transmit the ability to express delayed-type hypersensitivity and cell mediated immunity from sensitized donors to nonimmnune recipients. In this study, we determined the efficacy of TF as immunotherapy to treat experimental glioblastoma. We used TF obtained from immunized swine. We evaluated different doses of intratumoral TF (product of 4x10(6), 8x10(5) and 1.6x10(5) cells). The best dose (product of 4x10(6) cells) of TF was also combined with carmustine for experimental therapy in rats with C6 malignant glioma. Modifications in peripheral blood T lymphocyte counts ( CD2+, CD4+, CD8+ and NK) were evaluated by flow cytometry. Cytokine expression in the tumour was assessed by RT-PCR and apoptosis was evaluated using the sub G0 method. Intratumoral TF reduced significantly the tumour size, and increased CD2+, CD4+, CD8+ and NK cell counts, it also increased the percentage of apoptotic tumour cells and the percentage of tumour tissue expressing Th1 cytokines. We observed an additive antitumoral effect when TF was combined with chemotherapy.

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    • "Immunomodulation by Transferon® has been demon‐ strated by restoration of iNOS expression in a mouse model of tuberculosis, provoking inhibition of bacterial proliferation and significant increase of DTH [17] Transferon® also in‐ duces mRNA expression and IFN-γ secretion in peripheral blood mononuclear cells (PBMC) in animals with experimental glioma when compared with non-treated animals. [18] Due to Transferon® induces a Th1 response a clinical study comparing acyclovir treatment and Transferon® during human herpes virus infection was conducted; in that study patients treated with Transferon® had low incidence of clinical complications, better pain control, and also IFN-g was significant increased in serum when compared with patients treated on‐ ly with acyclovir. [19] Then, our group conducted a second clinical trial to evaluate immu‐ nological data and clinical outcome of patients with HSK treated with acyclovir or acyclovir and Transferon® as adjuvant therapy in patients with herpetic keratitis. "
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    • "Similar results were recorded in murine tuberculosis treated with TF and antibiotics (Fabre et al. 2004). Th1-inducing effect of TF was also confirmed in anti-tumour immunotherapy (Pineda et al. 2005). The serum concentration of IL-5 in mice infected with E. multilocularis and treated with TF or combination of TF +ABZ in our study was inhibited till the end of the experiment. "
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