Seasonal variations of a battery of biomarkers and physiological indices for the mussel Mytilus galloprovincialis transplanted into the northwest Mediterranean Sea

Université du Havre, El Havre, Haute-Normandie, France
Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology (Impact Factor: 2.3). 08/2004; 138(4):411-27. DOI: 10.1016/j.cca.2004.04.009
Source: PubMed


Seasonal variations of six mussel (Mytilus galloprovincialis) biomarkers at two sites in the Mediterranean Sea were compared with physiological indices (condition, growth and gonad maturation), environmental parameters (temperature, salinity and turbidity), and chemical contamination levels. The basal levels of acetylcholinesterase (AChE), DNA adducts, benzo[a]pyrene hydroxylase (BPH), heat-shock proteins (HSP70), metallothioneins (MT) and P-glycoprotein (P-gp)-mediated multixenobiotic resistance (MXR) were estimated as early warning signals in caged mussels sampled at Carteau (native site) and La Fourcade (transplantation site) over a 2-year period. The Carteau and La Fourcade mussels have specific chemical contamination profiles but a similar range of values. For example, both are highly contaminated by heavy metals (201 and 258.4 mg kg(-1) dw, respectively) and considered as moderately impacted for polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). However, contamination levels at Carteau are twice as high for PAHs (101.5 mg g(-1) dw) and PCBs (90.2 mg g(-1) dw) than La Fourcade. The seasonal contamination trend at Carteau showed six-fold higher levels of pyrolytic pollutants in winter. Although few tissue lesions were detected in individuals studied at either site, greater parasitic infestation was observed at Carteau. The results of findings from the two Mediterranean pilot studies support the adaptability of transplanted mussels to be used as biomarkers and to establish physiological endpoints for chemical contaminant exposure.

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    • "This could be related to a shifted reproduction, reflected by the variation schema of the gonad and hepatosomatic index in comparison with the reference population. Indeed, authors describe a decrease in GI in mussels from the area impacted by effluents from harbour activities (Amiard-Triquet and Amiard, 2012) this leads to disturb or even shift spawning periods (Bodin et al., 2004). "
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    ABSTRACT: In this study, we are interested in spatial and temporal variations of the biological and physiological responses of mussels collected from contrasting marine sites regarding their levels of pollution. We measured both the conditions indices and the enzymatic biomarker expression: acetylcholinesterase (AChE), catalase (CAT) and glutathione S-transferase (GST) activity. The enzymatic biomarkers were chosen because they respond to environmental stress. Results show a significant interactions between biomarker variations and conditions indices in the industrial harbor site throughout the seasons. But no significant changes in the reference site. Furthermore, we classified the sites along the seasons according to their potential ecotoxicity, calculated based on the sum of the normalised values of the biomarkers. The results show a very high biomarker index in the impacted site with irregular changes between seasons. This biomarker index is therefore a valuable tool that could be used to classify the toxic potential of coastal sites. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Marine Pollution Bulletin 04/2015; 95(1). DOI:10.1016/j.marpolbul.2015.03.041 · 2.99 Impact Factor
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    • "In the present study, higher condition index values were observed in summer in comparison to winter (Fig. 3c). These results are in agreement with previous studies in mussels and oysters (Bodin et al., 2004; Soletchnik et al., 2006). Interestingly, significant differences between the reference and transplantation sites were observed in summer, but not in winter. "
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    • "The use of biochemical biomarkers has proven especially useful in assessing and evaluating the effects of chemical compounds on the health and integrity of aquatic organisms. Numerous authors have underlined the importance of measuring multiple biochemical biomarkers at the same time in the same organisms, to evaluate the effects of environmental contaminants (Cajaraville et al., 2000; Romeo et al., 2003; Bodin et al., 2004; Chora et al., 2008; McDonagh and Sheehan, 2008; Tedesco et al., 2008, 2012; Schmidt et al., 2013; Pedriali et al., 2013; Sellami et al., 2014a,b). Interactions between chemicals and aquatic organisms often occur initially on the molecular scale. "
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