Seasonal variations of a battery of biomarkers and physiological indices for the mussel Mytilus galloprovincialis transplanted into the northwest Mediterranean Sea

Université du Havre, El Havre, Haute-Normandie, France
Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology (Impact Factor: 2.3). 08/2004; 138(4):411-27. DOI: 10.1016/j.cca.2004.04.009
Source: PubMed

ABSTRACT Seasonal variations of six mussel (Mytilus galloprovincialis) biomarkers at two sites in the Mediterranean Sea were compared with physiological indices (condition, growth and gonad maturation), environmental parameters (temperature, salinity and turbidity), and chemical contamination levels. The basal levels of acetylcholinesterase (AChE), DNA adducts, benzo[a]pyrene hydroxylase (BPH), heat-shock proteins (HSP70), metallothioneins (MT) and P-glycoprotein (P-gp)-mediated multixenobiotic resistance (MXR) were estimated as early warning signals in caged mussels sampled at Carteau (native site) and La Fourcade (transplantation site) over a 2-year period. The Carteau and La Fourcade mussels have specific chemical contamination profiles but a similar range of values. For example, both are highly contaminated by heavy metals (201 and 258.4 mg kg(-1) dw, respectively) and considered as moderately impacted for polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). However, contamination levels at Carteau are twice as high for PAHs (101.5 mg g(-1) dw) and PCBs (90.2 mg g(-1) dw) than La Fourcade. The seasonal contamination trend at Carteau showed six-fold higher levels of pyrolytic pollutants in winter. Although few tissue lesions were detected in individuals studied at either site, greater parasitic infestation was observed at Carteau. The results of findings from the two Mediterranean pilot studies support the adaptability of transplanted mussels to be used as biomarkers and to establish physiological endpoints for chemical contaminant exposure.

Download full-text


Available from: Nathalie Bodin, Sep 26, 2015
48 Reads
    • "This could be related to a shifted reproduction, reflected by the variation schema of the gonad and hepatosomatic index in comparison with the reference population. Indeed, authors describe a decrease in GI in mussels from the area impacted by effluents from harbour activities (Amiard-Triquet and Amiard, 2012) this leads to disturb or even shift spawning periods (Bodin et al., 2004). "
    [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we are interested in spatial and temporal variations of the biological and physiological responses of mussels collected from contrasting marine sites regarding their levels of pollution. We measured both the conditions indices and the enzymatic biomarker expression: acetylcholinesterase (AChE), catalase (CAT) and glutathione S-transferase (GST) activity. The enzymatic biomarkers were chosen because they respond to environmental stress. Results show a significant interactions between biomarker variations and conditions indices in the industrial harbor site throughout the seasons. But no significant changes in the reference site. Furthermore, we classified the sites along the seasons according to their potential ecotoxicity, calculated based on the sum of the normalised values of the biomarkers. The results show a very high biomarker index in the impacted site with irregular changes between seasons. This biomarker index is therefore a valuable tool that could be used to classify the toxic potential of coastal sites. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Marine Pollution Bulletin 04/2015; 95(1). DOI:10.1016/j.marpolbul.2015.03.041 · 2.99 Impact Factor
  • Source
    • "In the present study, higher condition index values were observed in summer in comparison to winter (Fig. 3c). These results are in agreement with previous studies in mussels and oysters (Bodin et al., 2004; Soletchnik et al., 2006). Interestingly, significant differences between the reference and transplantation sites were observed in summer, but not in winter. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Contaminant effects on defence responses of ecologically and economically important organisms, such as the Pacific oyster Crassostrea gigas, are likely to influence their ability to resist infectious diseases, particularly at the young stages. The aim of this study was to explore the potential relationships between organic contaminants accumulated in the soft tissues of juvenile oysters, defence responses and physiological condition. Oysters were transplanted during summer and winter periods in different sites in the Marennes-Oléron Bay, the first area of oyster production in France, and in the Gironde Estuary, the biggest estuary in Occidental Europe. Amongst the battery of biochemical and physiological biomarkers applied in the present work [superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), malondyaldehyde (MDA), catecholase, laccase and lysozyme in gills, digestive glands, mantle and haemolymph, glycogen, proteins and lipids in the digestive gland and the condition index at the whole-organism level], MDA and lysozyme in the digestive gland and SOD, GPx and laccase in plasma contributed in order to significantly discriminate the sites in which oysters bioaccumulated different levels of heavy polycyclic aromatic hydrocarbons (HPAHs), polychlorobiphenyls (PCBs), polybromodiphenylethers (PBDEs), dichlorodiphenyltrichloroethanes (DDTs) and lindane. These results strengthen the hypothesis that it is possible to differentiate sites depending on their contamination levels and biological effects by carrying out studies with transplanted juvenile oysters. In addition, correlations were found between antioxidant and immune-defence responses, and PAH and DDT body burdens in the first area of oyster production in France (the Marennes-Oléron Bay) and where considerable oyster mortalities have been reported. This result suggests that the presence of organic chemical contaminants in the Marennes-Oléron Bay may influence defence responses in juveniles of C. gigas, and, therefore, could influence their ability to resist infectious diseases.
    Science of The Total Environment 02/2015; 514. DOI:10.1016/j.scitotenv.2014.10.050 · 4.10 Impact Factor
  • Source
    • "The use of biochemical biomarkers has proven especially useful in assessing and evaluating the effects of chemical compounds on the health and integrity of aquatic organisms. Numerous authors have underlined the importance of measuring multiple biochemical biomarkers at the same time in the same organisms, to evaluate the effects of environmental contaminants (Cajaraville et al., 2000; Romeo et al., 2003; Bodin et al., 2004; Chora et al., 2008; McDonagh and Sheehan, 2008; Tedesco et al., 2008, 2012; Schmidt et al., 2013; Pedriali et al., 2013; Sellami et al., 2014a,b). Interactions between chemicals and aquatic organisms often occur initially on the molecular scale. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Anthracene (ANT) and permethrin (PER) are two of the more toxic compounds reaching the marine environment. This study aimed to determine the impact of these molecules on Venerupis decussate, an economically important species cultured on the Tunisian coast. Shell structure and its possible transformation upon exposure to the two contaminants were studied by X-ray diffraction and gravimetric analyses. Results revealed a phase transition in shell composition from aragonite to calcite after PER exposure, to a mixture of PER and ANT (Mix) but not for ANT alone. Catalase (CAT), superoxide dismutase (SOD) and glutathione transferase (GST) activities were determined in digestive gland and gills after exposure to ANT, PER and Mix to assess the impact of the contamination on the oxidative status of V. decussate. Enzyme activities increased in the digestive gland after PER treatment and in the gills after ANT treatment. PER exposure significantly reduced the levels of free thiols and increased levels of carbonylated proteins in the digestive gland, as compared to controls. In contrast, ANT exposure significantly reduced free thiols and increased the number of carbonylated proteins in the gills. Mix induced additive effects as measured by both enzymatic and proteomic approaches. The present study suggests that PER has a strong effect on shell structure; that PER and ANT exposure generate compound-dependent oxidative stress in the tissues of V. decussate and that a mixture of the two compounds has synergistic effects on biochemical response.
    Aquatic Toxicology 11/2014; 158. DOI:10.1016/j.aquatox.2014.10.020 · 3.45 Impact Factor
Show more