Article
Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell lines and a trophoblast/choriocarcinoma cell hybrid.
Department of Anatomy, Technical University of Aachen, Germany.
Cancer Genetics and Cytogenetics (impact factor:
1.39).
01/2000;
116(1):16-22.
pp.16-22
Source: PubMed
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Citations (0)
- Cited In (1)
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Article: The first trimester human trophoblast cell line ACH-3P: a novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations--TNF-alpha stimulates MMP15 expression.
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ABSTRACT: The trophoblast compartment of the placenta comprises various subpopulations with distinct functions. They interact among each other by secreted signals thus forming autocrine or paracrine regulatory loops. We established a first trimester trophoblast cell line (ACH-3P) by fusion of primary human first trimester trophoblasts (week 12 of gestation) with a human choriocarcinoma cell line (AC1-1). Expression of trophoblast markers (cytokeratin-7, integrins, matrix metalloproteinases), invasion abilities and transcriptome of ACH-3P closely resembled primary trophoblasts. Morphology, cytogenetics and doubling time was similar to the parental AC1-1 cells. The different subpopulations of trophoblasts e.g., villous and extravillous trophoblasts also exist in ACH-3P cells and can be immuno-separated by HLA-G surface expression. HLA-G positive ACH-3P display pseudopodia and a stronger expression of extravillous trophoblast markers. Higher expression of insulin-like growth factor II receptor and human chorionic gonadotropin represents the basis for the known autocrine stimulation of extravillous trophoblasts. We conclude that ACH-3P represent a tool to investigate interaction of syngeneic trophoblast subpopulations. These cells are particularly suited for studies into autocrine and paracrine regulation of various aspects of trophoblast function. As an example a novel effect of TNF-alpha on matrix metalloproteinase 15 in HLA-G positive ACH-3P and explants was found.BMC Developmental Biology 02/2007; 7:137. · 2.79 Impact Factor
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Keywords
AC1-specific chromosomal marker add(X)(q26)
choriocarcinoma/trophoblast hybrid ACH1P
conventional cytogenetic banding techniques
detailed characterization
first report
HGPRT-defective choriocarcinoma cell line AC1-1
HGPRT-defective mutant clones AC1-1
hybrid cells ACH1P
karyotypes
parental choriocarcinoma cell line JEG-3
primary male trophoblast cells
trophoblastic origin
underlying genetic mechanisms
unique cell culture model
