Human platelet antigen frequencies of platelet donors in the French population determined by polymerase chain reaction with sequence-specific primers
To prevent human platelet alloimmunization, Blood Transfusion Centres have to develop a strategy close to the erythrocytes' one. The first step of this strategy is to perform the HPA typing of donors with an accurate method. We applied the PCR-SSP to type 800 platelet donors in the HPA-1 and HPA-5 systems and 350 in the HPA-2 and HPA-3 ones. This study reports the human platelet antigen frequencies of four platelet-specific alloantigen systems in the French population. The results are quite similar to those currently published for Caucasian population frequencies. Low prevalences are observed for the HPA-1b, (2%), HPA-2b (0.6%) and HPA-5b (2%) groups. Furthermore, this study confirms the need to type donors and recipients in the HPA-1 system at least, in case of post-transfusion pupura and platelet refractoriness to platelet transfusion therapy.
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- "We cannot conclude from the testing of random donors that there were no false-positive results in the OD > 0´6 region. However, if there were false-positives, their number cannot have been high, as the observed frequency of HPA-1a negatives (45/ 1862, 2´4%) was as expected from other studies (Kiefel et al., 1993; Simsek et al., 1993; Merieux et al., 1997; Williamson et al., 1998). When compared with other HPA-1a phenotyping assays (Forsberg et al., 1995; Bessos et al., 1996; Denomme et al., 1996; Mohabir & Porter, 1997; Procter et al., 1998), the current test has undergone extensive validation, with almost 2000 samples, of which 80 were HPA-1a negative. "
ABSTRACT: A simplified method for large-scale HPA-1a phenotyping of platelets was developed for use in an antenatal screening programme for fetomaternal alloimmune thrombocytopenia (FMAIT). The test was based on the MAIPA assay, which was modified for antigen-typing with a well-characterized anti-HPA-1a reagent. The resulting assay gave reliable results, was inexpensive and allowed testing of large batches using semiautomated equipment.Transfusion Medicine 04/1994; 4(1):21-4. · 1.65 Impact Factor
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ABSTRACT: INTRODUCTION: By analogy, existence of erythrocyte antigens, classified in certain blood group systems, presence of specific alloantigens on platelets and leukocytes has been confirmed. CLINICAL SIGNIFICANCE OF PLATELET AND GRANULOCYTE ANTIGENS: Platelet and leukocyte antigens have a complex clinical significance, especially in hematology and blood transfusion. Immune response occurring after allosensibilization with platelet and granulocyte antigens is involved in pathogenesis of several clinical syndromes including: neonatal alloimmune thrombocytopenia, post-transfusion purpura, refractoriness to platelet transfusion, neonatal alloimmune neutropenia, transfusion related acute lung injury and chronic benign neutropenia in children. METHODS OF DETECTION: Application of various serological and molecular techniques enables phenotypization of platelet and granulocyte antigens and genomic analysis of DNA coding regions, providing determination of specific platelet and granulocyte alloantigen polymorphism. It confirms antigenic diversity of formed blood products.Medicinski pregled 54(7-8):357-63.
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ABSTRACT: BACKGROUND: The human platelet alloantigen system HPA-1 in the Egyptian population was examined by polymerase chain reaction using sequence-specific primers (PCR-SSP). The objectives of this study were to evaluate the allele frequency of HPA-1a and -1b in healthy Egyptian individuals and compare these with the international literature. Human platelet antigen (HPA) systems are associated with alloimmunization and organ transplantation rejection as well as the development of cardiovascular disease. Of the various HPA systems, HPA-1 specifically has been considered to be the most important antigenic system implicated in the Caucasian population. No study has yet examined this system in the Egyptian populations, however. We therefore investigated the allele frequency of the HPA-1 system in the Egyptian population. FINDINGS: To determine the allele frequency of the HPA-1a and -1b, we tested genomic DNAs from 206 healthy, unrelated Egyptian individuals using PCR-SSP. Our results showed that the 1a/1a genotype was the most predominant (59.22%) followed by 1a/1b (34.95%) and 1b/1b (5.83%) with allele frequencies for 1a and 1b of 0.77 and 0.23, respectively, in the population. CONCLUSION: As compared with other geographic groups, a relatively high allele frequency of the HPA-1b in the Egyptian population may indicate a higher risk of alloimmunization. This study is the first to investigate the allele frequency of the HPA-1 system in the Egyptian population and serves as an outline for future clinical research associated with platelet disorders in this group.
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