Lack of nucleotide variability in a beetle pest with extreme breeding

Department of Entomology, University of Wisconsin-Madison, USA.
Insect Molecular Biology (Impact Factor: 2.59). 05/1998; 7(2):197-200. DOI: 10.1046/j.1365-2583.1998.72064.x
Source: PubMed


The coffee berry borer beetle Hypothenemus hampei (Ferrari) (Curculionidae: Scolytinae) is the major insect pest of coffee and has spread to most of the coffee-growing countries of the world. This beetle also displays an unusual life cycle, with regular sibling mating. This regular inbreeding and the population bottlenecks occurring on colonization of new regions should lead to low levels of genetic diversity. We were therefore interested in determining the level of nucleotide variation in nuclear and mitochondrial genomes of this beetle worldwide. Here we show that two nuclear loci (Resistance to dieldrin and ITS2) are completely invariant, whereas some variability is maintained at a mitochondrial locus (COI), probably corresponding to a higher mutation rate in the mitochondrial genome. Phylogenetic analysis of the mitochondrial data shows only two clades of beetle haplotypes outside of Kenya, the proposed origin of the species. These data confirm that inbreeding greatly reduces nucleotide variation and suggest the recent global spread of only two inbreeding lines of this bark beetle.

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Available from: Lawrence Kirkendall, Oct 07, 2015
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    • "Those molecular techniques and others like PCR amplification followed by restriction endonuclease analysis (PCR–REN) [10] [19] and real-time PCR application using TaqMan [20] have been successfully developed to detect the resistance-associated mutation in other insect species. The Rdl gene was the main genetic marker used previously to study population genetic, heredity and reproductive behavior of the coffee berry borer [21] [22] [23], but it was not found before in the Colombian H. hampei insect popula- 0048-3575/$ -see front matter Ó 2010 Elsevier Inc. All rights reserved. "
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    ABSTRACT: The resistant Rdl allele for dieldrin insecticide was detected on the Hypothenemus hampei populations from Colombia using conventional PCR methods. Based on this sequence, a melting temperature (Tm) shift genotyping method that relies on allele-specific PCR is described for insecticide resistance-associated single nucleotide polymorphism (SNP) at the H. hampeiRdl gene. The method reported here uses GC-rich tails of unequal length attached to allele-specific primers containing 3′ terminal bases that correspond to SNP allelic variants. Specific PCR products are identified by inspection of a melting curve on a real-time PCR thermocycler using SYBR Green DNA binding dye. Resistant and susceptible alleles resulted in specific PCR products with Tm of 83.3±0.1°C and 86.0±0.2°C, respectively. The RdlTm-shift genotyping method is a new method to identify the Rdl gene in the coffee berry borer H. hampei, the principal pest of coffee that in general show low genetic diversity and very few genetic strategies for control of this pest have been developed. The method supplies a high-throughput tool for dieldrin resistance-associated SNP diagnostic in the coffee berry borer which will be useful for resistance-management strategies and as genetic marker in the colombian insect populations for genetics research.
    Pesticide Biochemistry and Physiology 07/2010; 97(3):204-208. DOI:10.1016/j.pestbp.2010.01.006 · 2.01 Impact Factor
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    • "These cyclical bottlenecks could have also erased or modified ancestral polymorphism. Such lack of information might explain the inability to resolve basal relationships among clades, as well as the incongruence between Cyt-b and COI markers indicated by the ILD test (Andreev et al., 1998; Gandolfi et al., 2001; Gorog et al., 2004). 4.3. "
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    ABSTRACT: Anopheles sundaicus s.l. is a malaria vector in coastal areas of Southeast Asia. Previous studies showed at least four distinct species within the complex. The present study investigated the phylogeography and the status of A. sundaicus s.l. populations from Cambodia, Thailand, Malaysia and Indonesia with regard to A. sundaicus s.s. from Sarawak, Malaysian Borneo and A. epiroticus in Vietnam and Thailand. Three lineages recovered by analyses of Cyt-b and COI (mtDNA) confirmed the presence of A. sundaicus s.s. in Malaysian Borneo, the distribution of A. epiroticus from southern Vietnam to peninsular Malaysia, and recognised a distinct form in Indonesia that is named A. sundaicus E. The phylogenetic and demographic analyses suggest that the three species were separated during the Early Pleistocene (1.8-0.78 Myr) and experienced bottlenecks followed by a genetic expansion in more recent times. Based on the results and knowledge of the biogeography of the area, we hypothesise that the combination of cyclical island and refugium creation was the cause of lineage isolation and bottleneck events during the Pleistocene.
    Infection Genetics and Evolution 08/2007; 7(4):484-93. DOI:10.1016/j.meegid.2007.02.003 · 3.02 Impact Factor
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    • "Any of these hypotheses might apply to all permanently inbred social systems such as spiders, psocids, naked mole rats, and some bark beetles (e.g. Mockford 1957; New 1985; Sherman et al. 1991; Kirkendall 1993; O'Riain et al. 1996; Andreev et al. 1998; Burland et al. 2002). Susceptibility to pathogens and inability to respond to changing environments (e.g., Slate and Pemberton 2002; Day et al. 2003; Schön et al. 2003; Pujolar et al. 2005) seem particularly relevant for social spiders due to their strong population subdivision, inbreeding , and high rates of colony turnover (Avilés 1993, 1997), all of which might erode genetic variability (Smith and Hagen 1996; Johannesen et al. 2002). "
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    ABSTRACT: Evolutionary "dead ends" result from traits that are selectively advantageous in the short term but ultimately result in lowered diversification rates of lineages. In spiders, 23 species scattered across eight families share a social system in which individuals live in colonies and cooperate in nest maintenance, prey capture, and brood care. Most of these species are inbred and have highly female-biased sex ratios. Here we show that in Theridiidae this social system originated eight to nine times independently among 11 to 12 species for a remarkable 18 to 19 origins across spiders. In Theridiidae, the origins cluster significantly in one clade marked by a possible preadaptation: extended maternal care. In most derivations, sociality is limited to isolated species: social species are sister to social species only thrice. To examine whether sociality in spiders represents an evolutionary dead end, we develop a test that compares the observed phylogenetic isolation of social species to the simulated evolution of social and non-social clades under equal diversification rates, and find that sociality in Theridiidae is significantly isolated. Because social clades are not in general smaller than their nonsocial sister clades, the "spindly" phylogenetic pattern-many tiny replicate social clades-may be explained by extinction rapid enough that a nonsocial sister group does not have time to diversify while the social lineage remains extant. In this case, this repeated origin and extinction of sociality suggests a conflict between the short-term benefits and long-term costs of inbred sociality. Although benefits of group living may initially outweigh costs of inbreeding (hence the replicate origins), in the long run the subdivision of the populations in relatively small and highly inbred colony lineages may result in higher extinction, thus an evolutionary dead end.
    Evolution 12/2006; 60(11):2342-51. DOI:10.1554/06-078.1 · 4.61 Impact Factor
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