Article

Simple Determination of Double-Bond Positions in Long-Chain Olefins by Cross-Metathesis

College of Pharmacy, Seoul National University, San 56-1, Shilim, Kwanak, Seoul 151-742 (Korea), Fax: (+82) 2-888-0649.
Angewandte Chemie International Edition (Impact Factor: 11.34). 08/2011; 50(36):8275-8. DOI: 10.1002/anie.201102634
Source: PubMed

ABSTRACT Cut, paste, and measure: The positions of double bonds in unsaturated long-chain compounds can be easily determined by chemical derivatization using a cross-metathesis reaction and chromatography-mass spectrometry. The produced olefins have distinct physicochemical properties suitable for LC/MS or GC/MS analysis that depend on the cross-metathesis partner used. This method is operationally simple and applicable at a sub-milligram scale.

0 Followers
 · 
116 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: End-of-life tire granulates were used in olefin metathesis reactions with ethene (ethenolysis); utilizing 0.0074 mmol of a Grubbs type ruthenium complex for 1.0 gram of tire granules leads to the isolation of ca. 0.5 g of organic solubles, which are primarily composed of oligomeric 1,4-cis isoprenes.
    Green Chemistry 01/2013; 15(2):315-319. DOI:10.1039/C2GC36417D · 6.85 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Lipidomics is an emerging field, where the structures, functions and dynamic changes of lipids in cells, tissues or body fluids are investigated. Due to the vital roles of lipids in human physiological and pathological processes, lipidomics is attracting more and more attentions. However, because of the diversity and complexity of lipids, lipid analysis is still full of challenges. The recent development of methods for lipid extraction and analysis and the combination with bioinformatics technology greatly push forward the study of lipidomics. Among them, mass spectrometry (MS) is the most important technology for lipid analysis. In this review, the methodology based on MS for lipid analysis was introduced. It is believed that along with the rapid development of MS and its further applications to lipid analysis, more functional lipids will be identified as biomarkers and therapeutic targets and for the study of the mechanisms of disease.
    International Journal of Molecular Sciences 06/2014; 15(6):10492-507. DOI:10.3390/ijms150610492 · 2.46 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In the course of our continuing search for bioactive constituents of Korean medicinal plants, we isolated five new oxylipins, chaenomic acid A-E (1-5), and six known ones (6-11) from the twigs of Chaenomeles sinensis. The structures of the new compounds (1-5) were determined by spectroscopic methods, including 1D and 2D NMR ((1)H and (13)C NMR, (1)H-(1)H COSY, HMQC, HMBC, and NOESY), olefin cross-metathesis, and LC/MS analysis. The known compounds (6-11) were identified by comparison of their spectroscopic data and specific optical rotation with the reported data. The isolated compounds (1-11) were tested for their inhibitory effects on nitric oxide production in lipopolysaccharide-activated murine microglial cells and for their cytotoxic activities against four human cancer cell lines (A549, SK-OV-3, A498, and HCT-15).
    Lipids 09/2014; 49(11). DOI:10.1007/s11745-014-3953-0 · 2.35 Impact Factor

Preview

Download
2 Downloads
Available from