[Simultaneous determination of 9 phenolic compounds in disinfectant by HPLC].
ABSTRACT A high performance liquid chromatography method was established for determination of 9 phenolic compounds in the disinfectant.
on a C18 (4.6 mm x 250 mm, 5 microm) chromatography column, use the methol-0.22% sodium heptane solution (pH 3.5) as mobile phase ladder washing, under the following conditions: flow rate 1.0 ml/min, detection wave 280 nm, and the column temperature 25 degrees C.
In this method, the linear range of each compound was 5 - 200 mg/L and the correlation coefficients was more than 0.9995. The detection limit was 1mg/L. The RSD was less than 4.8% and standard addition recovery varied from 97.6% to 108.2%.
This method was easy to operate, fast and with high sensitivity. It was suitable for routine analysis for disinfectant and can be used for the quality control of effective compounds in disinfectant.