Overexpression of KIAA0101 predicts poor prognosis in primary lung cancer patients.
ABSTRACT High expression of KIAA0101 (p15(PAF)/OEATC-1) which contains a proliferating cell nuclear antigen (PCNA)-binding motif, a key factor in DNA repair and/or apoptosis and cell cycle regulation, has been observed in a variety of human malignancies. The aim of this study was to observe the expression of KIAA0101 in human non-small-cell lung cancer (NSCLC), explore its clinicopathological significance and evaluate KIAA0101 expression as a potential prognostic marker. KIAA0101 transcript was found to be overexpressed in the great majority of lung cancers by semi-quantitative RT-PCR. A total of 357 NSCLCs were analyzed immunohistochemically on tissue microarrays. High-level KIAA0101 expression was observed in 33.9% (121 of 357 cases), and correlated with male gender (P<0.0001), tumor progression (pT status) (P=0.0008), lymph node metastasis (pN status) (P=0.0003), non-adenocarcinoma histological classification (P<0.0001), and smoking history (P<0.0001), but not with patient age or pleural invasion. Patients with tumors displaying high-level KIAA0101 expression showed significantly shorter survival (P<0.0001, log-rank test). Similarly, gender, pT status, pN status, pleural invasion, histological classification, and smoking history were significant prognostic markers in univariate Cox survival analysis. Importantly, high-level KIAA0101 expression was also identified as an independent prognostic factor by multivariate analysis (P=0.0320). These results provide additional information for determining postoperative adjuvant treatment of NSCLC.
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ABSTRACT: The ontogeny of the five queen mandibular gland semiochemicals that initiate and maintain the retinue behavior of worker honeybees was investigated by quantitative splitless capillary gas chromatography. No detectable pheromone is present at the time of eclosion, but decenoic acid levels build up rapidly during the first week of the queen's life. Two aromatic components attain detectable levels later, with the more plentiful methylp-hydroxybenzoate preceding the 4-hydroxy-3-methoxyphenylethanol. Pheromone levels are maximal in mature, mated, laying queens. The ratio of (R,E)-(–)-9-hydroxy-2-decenoic acid to the (S,E)-(+) enantiomer increases with the age of the queen. Pheromone levels in queen mandibular glands are largely unaffected by queen banking, restraint with workers in mailing cages, and limited storage on dry ice. All major body parts of typical queens, especially the head and legs, have sufficient mandibular exudate to be highly attractive to worker bees.Journal of Chemical Ecology 02/1990; 16(3):851-860. · 2.46 Impact Factor
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ABSTRACT: The mechanism of hydrocarbon biosynthesis was examined in the fleshfly Sarcophaga crassipalpis, the blowfly Phormia regina, the German cockroach Blattella germanica, the house cricket Acheta domesticus, the Mormon cricket Anabrus simplex, the dampwood termite Zootermopsis nevadensis and compared to the house fly, Musca domestica. Microsomal preparations from each species readily converted [9,10-3H2]octadecanal (18:0 Ald) to heptadecane. NADPH and O2 were required for enzymic activity in all cases, and very little hydrocarbon was formed under anaerobic conditions. Radio-GLC analyses of the head space gas formed from the metabolism of [1-14C]18:0 Ald by microsomes from M. domestica, S. crassipalpis, P. regina and B. germanica clearly showed that 14CO2 and not 14CO was formed. Quantitation of the products from the metabolism of [1-14C] and [9,10-3H2]18:0 Ald in microsomes from M. domestica, P. regina, S. crassipalpis, B. germanica and Z. nevadensis showed that an approximate ratio of 14CO2/[3H] heptadecane was formed. The data support a mechanism in which the aldehyde is converted to hydrocarbon and CO2 in insects.Insect Biochemistry and Molecular Biology. 01/1996;
Article: Mimicry and Deception in Pollination11/2003; 15:259-278.