Article

Radiation decreases murine small intestinal HCO3- secretion.

Vascular Medicine Research, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts, USA. K. Zhang
International Journal of Radiation Biology (Impact Factor: 1.84). 06/2011; 87(8):878-88. DOI: 10.3109/09553002.2011.583314
Source: PubMed

ABSTRACT While secretagogue-induced diarrhea is rich in chloride (Cl(-)) and bicarbonate (HCO(3) (-)) anions, little is known about diarrhea or its anionic composition following irradiation. We performed studies to characterize the differences between cyclic adenosine monophosphate (cAMP)-stimulated anion secretions in irradiated and non-irradiated mice.
HCO(3) (-) secretion was examined in basal, cAMP-stimulated, and irradiated jejunal tissues from BALB/c (Bagg albino) mice. The abdomens of the mice were γ-irradiated using a caesium-137 source.
Ussing-chamber experiments performed in an HCO(3)(-)-containing, Cl(-)-free solution on the bath side showed inhibition of HCO(3)(-) in irradiated mice. Non-irradiated mice exhibited bumetanide-sensitive and insensitive current, while irradiated mice displayed bumetanide-sensitive current. pH-stat experiments showed inhibition of basal and cAMP-stimulated HCO(3)(-) secretions in irradiated mice. Immunohistochemistry and Western blot analysis displayed a sodium-bicarbonate cotransporter expression in the villus and not the crypt of non-irradiated mice, while its expression and protein levels decreased in irradiated mice.
Anion secretions in irradiated mice, being primarily Cl(-) and minimally HCO(3)(-), differ from that of secretagogue-induced anion secretions. Understanding anion loss will help us correct electrolyte imbalances, while reduced HCO(3)(-) secretion in the upper-gastrointestinal tract might also have implications for irradiation-induced nausea and vomiting.

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