Article

Tryptase Enzyme Activity Is Correlated with Severity of Chronic Obstructive Pulmonary Disease

First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
The Tohoku Journal of Experimental Medicine (Impact Factor: 1.28). 07/2011; 224(3):179-87. DOI: 10.1620/tjem.224.179
Source: PubMed

ABSTRACT Tryptic enzymes, including tryptase, a signature enzyme in mast cells, are involved in the pathogenesis of chronic obstructive pulmonary disease (COPD), a chronic inflammatory airway disease. However, the relationship between tryptase enzyme activity and COPD remains to be investigated. We therefore measured the enzyme activity and immunoreactivity of tryptase in the sputum and plasma of COPD patients in the present study. The results showed that tryptase enzyme activity in the sputum of severe COPD patients (FEV(1)s being recorded at ≤ 30% prediction values) was 3.4 times greater than that in patients with mild COPD (FEV(1)s being recorded at ≥ 80% of predicted values), whereas tryptic activity was 2.0 times higher in the severe COPD patients than in mild COPD patients. Moreover, tryptase enzyme activity, but not tryptic enzyme activity, was significantly elevated in the plasma of severe COPD patients compared with that of mild COPD patients. The level of immunoreactive tryptase was 1.9 times higher in the sputum of the severe COPD patients at admission than that at remission stage. We also employed a rat model of cigarette smoke-induced COPD. After 36 weeks of daily challenges with cigarette smoke, a well-established risk factor of COPD, tryptic and tryptase activities in the bronchoalveolar lavage fluid were elevated 1.5 and 2.6 times, respectively. These results indicate that smoking induces tryptase enzyme activity in the airway. In conclusion, tryptase enzyme activity is markedly increased in sputum and plasma of severe COPD patients. Enhanced tryptase enzyme activity may contribute to the pathogenesis of COPD.

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Available from: Changting Liu, Aug 02, 2015
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    • "Kalenderian et al. [19] [20] found that the levels of mast cell mediators, such as histamine and tryptase, are considerably elevated in BALF from smokers. The importance of mast cells is further supported by the fact that mast cell tryptase activity is correlated with the severity of COPD [21], and in COPD patients an accumulation of mast cells in the airways has been observed [22]. Mast cells located here could be exposed to inhaled environmental challenges, and mast cell activation results in the coordinated release of proinflammatory mediators into the surrounding tissue; activation of this cell type may result in pathology associated with chronic inflammatory stimuli [23] [24]. "
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