Article

Natural product-like synthetic libraries.

Institute of Chemical and Engineering Sciences, Organic Chemistry, 11 Biopolis Way, Helios, Singapore 138667, Singapore.
Current opinion in chemical biology (impact factor: 8.3). 06/2011; 15(4):516-22. DOI:10.1016/j.cbpa.2011.05.022
Source: PubMed

ABSTRACT There is a paucity of chemical matter suitably poised for effective drug development. Improving the quality and efficiency of research early on in the drug discovery process has been a long standing objective for the drug industry and improvements to the accessibility and quality of compound screening decks might have a significant and positive impact. In the absence of specific molecular information that can be modeled and used predicatively we are far from identifying which small molecules are most relevant to emerging biological targets such as protein-protein interactions. Natural products have been historically successful as an entry point for drug discovery and recently screening libraries are being synthesized to emulate natural product like features.

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    Article: Interaction of Multimicrobial Synthetic Inhibitor 1,2- Bis(2-Benzimidazolyl)-1,2-Ethanediol with Serum Albumin: Spectroscopic and Computational Studies
    Nayana Kamtekar, Anita Pandey, Neeraj Agrawal, Raghuvir R S Pissurlenkar, Mohanish Borana, Basir Ahmad
    [show abstract] [hide abstract]
    ABSTRACT: The molecule, 1,2-Bis(2-benzimidazolyl)-1,2-ethanediol (BBE) is known to act as a selective inhibitor of poliovirus, rhinovirus, Candida albicans, several bacterial species, and is easily synthesized by Phillips reaction. The interaction of BBE with BSA and the effects of its binding on the conformation and unfolding/refolding pathways of the protein were investigated using multispectroscopic techniques and molecular modeling. The binding studies indicate that BSA has one high affinity BBE binding site with association constant 6.0260.05610 4 M 21 at 298 K. By measuring binding at different temperatures, we determined the changes in enthalpy (DH = 215.1362.15 kJ mol 21), entropy (DS = 40.8767.25 J mol 21 K 21) and free energy (DG = 26.7861.02) of interaction, which indicate that the binding was spontaneous and both enthalpically and entropically driven. Based on molecular modeling and thermodynamic parameters, we proposed that the complex formation involved mainly hydrophilic interaction such as hydrogen bonding between hydroxyl groups of ethane-1,2-diol fragment with Tyr410 and benzimidazole sp 2 nitrogen atom with Ser488 and hydrophobic interaction between phenyl ring of one benzimidazole of the ligand and hydrophobic residues namely, Ile387, Cys391, Phe402, Val432 and Cys437. The sequential unfolding mechanism of BSA, site-specific marker displacement experiments and molecular modeling showed that the molecule preferably binds in subdomain IIIA. The BBE binding to BSA was found to cause both secondary and tertiary structural alterations in the protein as studied by intrinsic fluorescence, near-UV and far-UV circular dichroism results. The unfolding/ refolding study showed that BBE stabilized native to intermediate states (N=I) transition of the protein by ,2 kJ mol 21 without affecting the intermediate to unfolded states (I=U) transition and general mechanism of unfolding of BSA.
    PLoS ONE 01/2013; · 4.09 Impact Factor
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Keywords

accessibility
 
biological targets
 
chemical matter suitably
 
compound screening decks
 
drug discovery
 
drug discovery process
 
drug industry
 
effective drug development
 
entry point
 
natural product
 
Natural products
 
screening libraries
 
small molecules
 
specific molecular information