Solution NMR study of integral membrane proteins

Experimental Therapeutics Centre, Agency for Science, Technology and Research, Singapore 138669, Singapore.
Current opinion in chemical biology (Impact Factor: 7.65). 06/2011; 15(4):560-9. DOI: 10.1016/j.cbpa.2011.05.025
Source: PubMed

ABSTRACT Signals between a cell and its environment are often transmitted through membrane proteins; therefore, many membrane proteins, including G protein-coupled receptors (GPCRs) and ion channels, are important drug targets. Structural information about membrane proteins remains limited owing to challenges in protein expression, purification and the selection of membrane-mimicking systems that will retain protein structure and function. This review describes recent advances in solution NMR applied to the structural study of integral membrane proteins. The examples herein demonstrate that solution NMR spectroscopy will play a unique role not only in structural analysis, but also drug discovery of membrane proteins.

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    • "The vast majority of solution-state NMR studies of MPs to date have been performed in detergent solutions (http://; Kang and Li 2011). "
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    ABSTRACT: Solution-state nuclear magnetic resonance studies of membrane proteins are facilitated by the increased stability that trapping with amphipols confers to most of them as compared to detergent solutions. They have yielded information on the state of folding of the proteins, their areas of contact with the polymer, their dynamics, water accessibility, and the structure of protein-bound ligands. They benefit from the diversification of amphipol chemical structures and the availability of deuterated amphipols. The advantages and constraints of working with amphipols are discussed and compared to those associated with other non-conventional environments, such as bicelles and nanodiscs.
    Journal of Membrane Biology 03/2014; 247(9-10). DOI:10.1007/s00232-014-9654-z · 2.17 Impact Factor
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    • "of membrane proteins (MPs) give access to such important biological information as their secondary and tertiary structure, their dynamics, the way they recognize their ligands, etc. Traditionally, these studies are performed in aqueous detergent solutions (Kang and Li 2011). However, the destabilizing character of detergents, the high concentrations of them that is required for NMR experiments, the relatively high temperatures at which those are carried out and their long duration combine to make it difficult to prepare stable enough MP samples. "
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    ABSTRACT: Amphipols are short amphipathic polymers that can substitute for detergents at the hydrophobic surface of membrane proteins (MPs), keeping them soluble in the absence of detergents while stabilizing them. The most widely used amphipol, known as A8-35, is comprised of a polyacrylic acid (PAA) main chain grafted with octylamine and isopropylamine. Among its many applications, A8-35 has proven particularly useful for solution-state NMR studies of MPs, for which it can be desirable to eliminate signals originating from the protons of the surfactant. In the present work, we describe the synthesis and properties of perdeuterated A8-35 (perDAPol). Perdeuterated PAA was obtained by radical polymerization of deuterated acrylic acid. It was subsequently grafted with deuterated amines, yielding perDAPol. The number-average molar mass of hydrogenated and perDAPol, ~4 and ~5 kDa, respectively, was deduced from that of their PAA precursors, determined by size exclusion chromatography in tetrahydrofuran following permethylation. Electrospray ionization-ion mobility spectrometry-mass spectrometry measurements show the molar mass and distribution of the two APols to be very similar. Upon neutron scattering, the contrast match point of perDAPol is found to be ~120 % D2O. In (1)H-(1)H nuclear overhauser effect NMR spectra, its contribution is reduced to ~6 % of that of hydrogenated A8-35, making it suitable for extended uses in NMR spectroscopy. PerDAPol ought to also be of use for inelastic neutron scattering studies of the dynamics of APol-trapped MPs, as well as small-angle neutron scattering and analytical ultracentrifugation.
    Journal of Membrane Biology 03/2014; 247(9-10). DOI:10.1007/s00232-014-9656-x · 2.17 Impact Factor
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    ABSTRACT: Dengue virus causes serious diseases affecting people in tropical and sub-tropical regions. The nonstructural (NS) protein 2B is an integral membrane protein and important for the regulation of viral protease NS3, which is significant for virus replication. The NS2B-NS3 complex is an important drug target for treating dengue fever. However, little is known about the structure of NS2B in its entirety. Herein, we describe the expression and purification of this integral membrane protein from cell membrane and inclusion bodies of Escherichia coli cells. The initial nuclear magnetic resonance (NMR) and circular dichroism (CD) results indicate that the purified protein adopts alpha-helical structures in LMPG and TDPC micelles.
    Protein Expression and Purification 08/2011; 80(2):169-75. DOI:10.1016/j.pep.2011.08.008 · 1.51 Impact Factor
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