Structural and Functional Modulation of Early Healing of Full-thickness Superficial Digital Flexor Tendon Rupture in Rabbits by Repeated Subcutaneous Administration of Exogenous Human Recombinant Basic Fibroblast Growth Factor
The present study was designed to investigate the effects of basic fibroblast growth factor on the healing of the acute phase of complete superficial digital flexor tendon rupture in rabbits. A total of 40 skeletally mature female white New Zealand rabbits were randomly divided into 2 equal groups of injured treated and injured control. After tenotomy and surgical anastomosis, using a modified Kessler and running pattern, the injured legs were placed in casts for 14 days, and basic fibroblast growth factor was injected subcutaneously over the lesion on days 3, 7, and 10 after injury. The injured control rabbits received a normal saline injection in a similar protocol. The rabbits' weight, tendon diameter, clinical signs, radiographs, and ultrasound scans were evaluated weekly. The rabbits were killed 28 days after injury, and the tendons were evaluated at the macroscopic, histopathologic, and ultrastructural levels and for biomechanical and the percentage of dry weight analysis. Treatment significantly reduced the diameter and increased the echogenicity and dry weight content and enhanced the maturation rate of the tenoblasts, fibrillogenesis, collagen fibril diameter, fibrillar density, tensile strength, and stiffness and stress of the injured tendons. Treatment with basic fibroblast growth factor was effective in restoring the morphologic and biomechanical properties of the injured superficial digital flexor tendon and could be valuable in clinical trial studies.
"Each of these can be used alone (monotherapy) or designed in combination with other components as poly-therapy  . One of the most important features that a tissue engineered bio-implant should exhibit is osteoinductivity usually provided by growth factors   . Osteoinduction is the process of differentiation of the mesenchymal stem cells (MSCs) into osteoprogenitor cells and ultimately into osteoblasts to form new bone  . "
[Show abstract][Hide abstract] ABSTRACT: Healing and regeneration of large bone defects leading to
non-unions is a great concern in orthopedic surgery. Since
auto- and allografts have limitations, bone tissue engineering
and regenerative medicine (TERM) has attempted to solve this
issue. In TERM, healing promotive factors are necessary to
regulate the several important events during healing. An ideal
treatment strategy should provide osteoconduction, osteoinduction,
osteogenesis, and osteointegration of the graft or biomaterials
within the healing bone. Since many materials have
osteoconductive properties, only a few biomaterials have
osteoinductive properties which are important for osteogenesis
and osteointegration. Bone morphogenetic proteins (BMPs)
are potent inductors of the osteogenic and angiogenic activities
during bone repair. The BMPs can regulate the production
and activity of some growth factors which are necessary for the osteogenesis. Since the introduction of BMP, it has
added a valuable tool to the surgeon’s possibilities and is
most commonly used in bone defects. Despite significant evidences
suggesting their potential benefit on bone healing,
there are some evidences showing their side effects such as
ectopic bone formation, osteolysis and problems related to
cost effectiveness. Bone tissue engineering may create a local
environment, using the delivery systems, which enables BMPs
to carry out their activities and to lower cost and complication
rate associated with BMPs. This review represented the most
important concepts and evidences regarding the role of BMPs
on bone healing and regeneration from basic to clinical application.
The major advantages and disadvantages of such biologic
compounds together with the BMPs substitutes are also
discussed. VC 2014 BioFactors, 00(00):000–000, 2014
"This multifunctional, 146 amino acid polypeptide has been shown to affect the proliferation, chemotaxis, and differentiation of mesoderm-derived cells  . Recent studies have identified it as a potential stimulator of angiogenesis, and this protein has been intensively studied in the wound healing processes of various animals   . Moreover, other proteins involved in the regulation of angiogenesis, such as vascular endothelial growth factor- A (VEGF-A), have been shown to play important roles in the remodeling of tendons and in their degenerative diseases . "
[Show abstract][Hide abstract] ABSTRACT: Physical exercise and massage are regarded as key factors in regulating tendon structure. However, information on the mechanism through which massage influences the structure and biology of a tendon is scarce. In this study, we attempted to define the impact of these two activities on rat tendons by using morphological and molecular techniques, determining the expression of VEGF-A, FGF-2, and CD34 in the tendons of rats subjected to 10 weeks of physical exercise (running) with massage of varied duration. The group of rats that was trained and massaged during the entire study was characterized by the highest expression of these markers, compared to the rats subjected to massage before training and to the control group subjected to physical exercises only. The greatest significant differences, compared to the control, were noted in the expression of all the studied markers at mRNA level, and in the case of VEGF-A, at protein level, in the third and fifth weeks of the experiment. The results of this study could point to the synergistic impact of simultaneous massage and physical exercise on the expression of angiogenesis markers in rat tendons.
BioMed Research International 05/2014; 2014:878095. DOI:10.1155/2014/878095 · 3.17 Impact Factor
"The samples obtained from the ICTs, ITTCs and ITTC-PDSs and their NCTs were weighed immediately after euthanasia, and freeze-dried (Helosicc, Ink, Co. London) until a constant weight was obtained and the percentage dry weight was then calculated according to the following equation: dry weight/wet weight × 100 , . "
[Show abstract][Hide abstract] ABSTRACT: Healing of large tendon defects is challenging. We studied the role of collagen implant with or without polydioxanone (PDS) sheath on the healing of a large Achilles tendon defect model, in rabbits. Sixty rabbits were divided into three groups. A 2 cm gap was created in the left Achilles tendon of all rabbits. In the control lesions, no implant was used. The other two groups were reconstructed by collagen and collagen-PDS implants respectively. The animals were clinically examined at weekly intervals and their lesions were observed by ultrasonography. Blood samples were obtained from the animals and were assessed for hematological analysis and determination of serum PDGF level, at 60 days post injury (DPI). The animals were then euthanized and their lesions were assessed for gross and histopathology, scanning electron microscopy, biomechanical testing, dry matter and hydroxyproline content. Another 65 pilot animals were also studied grossly and histopathologically to define the host implant interaction and graft incorporation at serial time points. The treated animals gained significantly better clinical scoring compared to the controls. Treatment with collagen and collagen-PDS implants significantly increased the biomechanical properties of the lesions compared to the control tendons at 60DPI (P<0.05). The tissue engineered implants also reduced peritendinous adhesion, muscle fibrosis and atrophy, and increased ultrasonographical echogenicity and homogenicity, maturation and differentiation of the collagen fibrils and fibers, tissue alignment and volume of the regenerated tissue compared to those of the control lesions (P<0.05). The implants were gradually absorbed and substituted by the new tendon. Implantation of the bioimplants had a significant role in initiating tendon healing and the implants were biocompatible, biodegradable and safe for application in tendon reconstructive surgery. The results of the present study may be valuable in clinical practice.
PLoS ONE 09/2013; 8(9):e73016. DOI:10.1371/journal.pone.0073016 · 3.23 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.