Article

Myoglobin-biomimetic electroactive materials made by surface molecular imprinting on silica beads and their use as ionophores in polymeric membranes for potentiometric transduction.

BIOMARK/ISEP, Instituto Superior de Engenharia do Porto, Porto, Portugal.
Biosensors & bioelectronics (impact factor: 5.43). 06/2011; 26(12):4760-6. DOI:10.1016/j.bios.2011.05.045 pp.4760-6
Source: PubMed

ABSTRACT Myoglobin (Mb) is among the cardiac biomarkers playing a major role in urgent diagnosis of cardiovascular diseases. Its monitoring in point-of-care is therefore fundamental. Pursuing this goal, a novel biomimetic ionophore for the potentiometric transduction of Mb is presented. It was synthesized by surface molecular imprinting (SMI) with the purpose of developing highly efficient sensor layers for near-stereochemical recognition of Mb. The template (Mb) was imprinted on a silane surface that was covalently attached to silica beads by means of self-assembled monolayers. First the silica was modified with an external layer of aldehyde groups. Then, Mb was attached by reaction with its amine groups (on the external surface) and subsequent formation of imine bonds. The vacant places surrounding Mb were filled by polymerization of the silane monomers 3-aminopropyltrimethoxysilane (APTMS) and propyltrimethoxysilane (PTMS). Finally, the template was removed by imine cleavage after treatment with oxalic acid. The results materials were finely dispersed in plasticized PVC selective membranes and used as ionophores in potentiometric transduction. The best analytical features were found in HEPES buffer of pH 4. Under this condition, the limits of detection were of 1.3 × 10(-6)mol/L for a linear response after 8.0 × 10(-7) mol/L with an anionic slope of -65.9 mV/decade. The imprinting effect was tested by preparing non-imprinted (NI) particles and employing these materials as ionophores. The resulting membranes showed no ability to detect Mb. Good selectivity was observed towards creatinine, sacarose, fructose, galactose, sodium glutamate, and alanine. The analytical application was conducted successfully and showed accurate and precise results.

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11 Jan 2013

Keywords

amine groups
 
analytical features
 
anionic slope
 
cardiac biomarkers
 
cardiovascular diseases
 
efficient sensor layers
 
imine cleavage
 
linear response
 
major role
 
oxalic acid
 
plasticized PVC selective membranes
 
potentiometric transduction
 
precise results
 
resulting membranes
 
self-assembled monolayers
 
silane monomers 3-aminopropyltrimethoxysilane
 
sodium glutamate
 
subsequent formation
 
urgent diagnosis
 
vacant places