Article

CREST maps somatic structural variation in cancer genomes with base-pair resolution

Department of Information Sciences, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Nature Methods (Impact Factor: 25.95). 06/2011; 8(8):652-4. DOI: 10.1038/nmeth.1628
Source: PubMed

ABSTRACT We developed 'clipping reveals structure' (CREST), an algorithm that uses next-generation sequencing reads with partial alignments to a reference genome to directly map structural variations at the nucleotide level of resolution. Application of CREST to whole-genome sequencing data from five pediatric T-lineage acute lymphoblastic leukemias (T-ALLs) and a human melanoma cell line, COLO-829, identified 160 somatic structural variations. Experimental validation exceeded 80%, demonstrating that CREST had a high predictive accuracy.

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