Study on the flocs poly-β-hydroxybutyrate production and process optimization in the bio-flocs technology system

Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029, China.
Bioresource Technology (Impact Factor: 4.49). 08/2011; 102(16):7599-602. DOI: 10.1016/j.biortech.2011.05.028
Source: PubMed


The bio-flocs technology (BFT) was applied in the sequencing batch reactor (SBR) to treat aquaculture wastewater for flocs poly-β-hydroxybutyrate (PHB) accumulation with alternant anaerobic and aerobic conditions. The statistical modeling approach was used to evaluate system performance and to optimize the flocs PHB yield at batch mode. The results show that all variables have significant impact on the response objective, as well as the interactions of the C/N ratio with the flocs biomass concentration (VSS) and anaerobic time, respectively. By process optimization, approximately 150-200 PHB/VSS (mg·g) of flocs PHB yield was achieved in the range of 4-7g/l of flocs biomass concentration, 15-18 of the C/N ratio and 50-85min of anaerobic time in the BFT systems. The results demonstrated that a suitable flocs PHB yield can be obtained via optimizing the ex-situ operating strategy, which have potential prebiotic value and practical implication for the sustainable aquaculture.

1 Follower
5 Reads
  • Source
    • "BFT system has been reported that the yield and accumulation of poly-␤hydroxybutyrate (PHB) under intermittent aeration are greater than those under continuous aeration (CA), where an aeration process involving a 0.5-h aeration/0.5-h non-aeration intermittent cycle has been proven to be the best (Liu et al., 2011; Ruan et al., 2011). For BFT used in the biological treatment of municipal and industrial wastewaters, studies have primarily focused on the physical properties of flocs that contribute to hindering flocs' settling and compactability (Nguyen et al., 2008). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Aquacultural solid waste from a recirculation aquaculture system was used as a substrate to produce heterotrophic bacteria in suspended growth reactors. The efficiency of nitrogen recycling under intermittent aeration (IA, 0.5-h aeration/0.5-h non aeration) and continuous aeration (CA) strategy was investigated. The nitrogen dynamics, biochemical composition of biofloc and efficiency of nitrification/denitrificaiton/ammonium assimilation of biofloc were determined. No significant differences were observed in the nitrogen recycling rate, crude protein and polysaccharides contents of biofloc between the IA and CA reactors. The energy used for intermittent aeration was almost one half of that for continuous aeration. IA strategy (0.5-h aeration/0.5-h non aeration) appears to be more effective to produce biofloc in aquaculture solid waste in reactors than CA strategy.
    Aquacultural Engineering 03/2014; 59. DOI:10.1016/j.aquaeng.2014.02.001 · 1.18 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Nitrate removal is essential for the sustainable operation of recirculating aquaculture system (RAS). This study evaluated the heterotrophic denitrification using poly(butylene succinate) as carbon source and biofilm carrier for RAS wastewater treatment. The effect of varied operational conditions (influent type, salinity and nitrate loading) on reactor performance and microbial community was investigated. The high denitrification rates of 0.53±0.19kgNO3(-)-Nm(-3)d(-1) (salinity, 0‰) and 0.66±0.12kgNO3(-)-Nm(-3)d(-1) (salinity, 25‰) were achieved, and nitrite concentration was maintained below 1mg/L. In addition, the existence of salinity exhibited more stable nitrate removal efficiency, but caused adverse effects such as excessive effluent dissolved organic carbon (DOC) and dissimilation nitrate reduce to ammonia (DNRA) activity. The degradation of PBS was further confirmed by SEM and FTIR analysis. Illumina sequencing revealed the abundance and species changes of functional denitrification and degradation microflora which might be the primary cause of varied reactor performance. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Bioresource Technology 06/2015; 192:603-610. DOI:10.1016/j.biortech.2015.06.021 · 4.49 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Background: Biodegradable polyesters are candidates for the development of environmental friendly plastics. Poly-β-hydroxybutyrate (PHB) is a type of polyester from the hydroxyalkanoates family, synthesized by bacteria as an intracellular material and accumulated as granules in the cytoplasm. Objectives: The aim of this study was to isolate Poly β-hydroxybutyrate over producing bacteria and optimize the production medium. Materials and Methods: A variety of PHB-accumulating bacterial strains were isolated from Kermanshah oil refinery sludge in Iran. Poly-β-hydroxybutyrate-producing bacterial strains were confirmed by the gas chromatography method. The strain with the highest rate of PHB production was selected. Use of sugar cane molasses, a by-product of the sugar refinery industry, was investigated for the production of PHB. Plackett-Burman statistical method was employed to obtain factors in cell growth and PHB production. Optimization by the Response Surface Method (RSM) was done via two carbon sources, glucose and molasses. Results: In the present study, 21 of 63 strains isolated from the refinery oil sludge produced PHB, seven of which were over producers. Poly-β-hydroxybutyrate production was analyzed by Sudan Black B staining, spectrophotometric and gas chromatography (GC) methods. The strain with the highest rate of PHB production was used to optimize the culture medium. Fifteen factors were analyzed in PHB production by the Plackett-Burman method to find the most effective factors. Five important factors were optimized by RSM. Molasses were used as a cheap source of carbon. The maximum PHB obtained from molasses was 6.62 g/L. The phenotypic and 16S rRNA biotyping tests led to the identification of the isolate as Bacillus coagulans. Conclusions: The results suggest that B. coagulans is a good candidate for the fermentative production of PHB.
    Jundishapur Journal of Microbiology 07/2015; 8(7):e16965. DOI:10.5812/jjm.16965v2 · 0.39 Impact Factor