Article

Gene expression profile of the regeneration epithelium during axolotl limb regeneration.

Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, USA.
Developmental Dynamics (impact factor: 2.54). 07/2011; 240(7):1826-40. DOI:10.1002/dvdy.22669 pp.1826-40
Source: PubMed

ABSTRACT Urodele amphibians are unique among adult vertebrates in their ability to regenerate missing limbs. The process of limb regeneration requires several key tissues including a regeneration-competent wound epidermis called the regeneration epithelium (RE). We used microarray analysis to profile gene expression of the RE in the axolotl, a Mexican salamander. A list of 125 genes and expressed sequence tags (ESTs) showed a ≥1.5-fold expression in the RE than in a wound epidermis covering a lateral cuff wound. A subset of the RE ESTs and genes were further characterized for expression level changes over the time-course of regeneration. This study provides the first large scale identification of specific gene expression in the RE.

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Keywords

adult vertebrates
 
axolotl
 
expression level changes
 
first large scale identification
 
lateral cuff wound
 
limb regeneration
 
limbs
 
Mexican salamander
 
profile gene expression
 
RE
 
regeneration epithelium
 
regeneration-competent wound epidermis
 
sequence tags
 
specific gene expression
 
time-course
 
Urodele amphibians
 
wound epidermis
 
≥1.5-fold expression