Article

Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels

Institut National de Santé et de Recherche Médicale (INSERM), U603, Paris, France.
Nature medicine (Impact Factor: 28.05). 06/2011; 17(7):893-8. DOI: 10.1038/nm.2394
Source: PubMed

ABSTRACT Uncovering principles that regulate energy metabolism in the brain requires mapping of partial pressure of oxygen (PO(2)) and blood flow with high spatial and temporal resolution. Using two-photon phosphorescence lifetime microscopy (2PLM) and the oxygen probe PtP-C343, we show that PO(2) can be accurately measured in the brain at depths up to 300 μm with micron-scale resolution. In addition, 2PLM allowed simultaneous measurements of blood flow and of PO(2) in capillaries with less than one-second temporal resolution. Using this approach, we detected erythrocyte-associated transients (EATs) in oxygen in the rat olfactory bulb and showed the existence of diffusion-based arterio-venous shunts. Sensory stimulation evoked functional hyperemia, accompanied by an increase in PO(2) in capillaries and by a biphasic PO(2) response in the neuropil, consisting of an 'initial dip' and a rebound. 2PLM of PO(2) opens new avenues for studies of brain metabolism and blood flow regulation.

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Available from: Mathieu Ducros, May 29, 2015
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