Effects of varicocelectomy on sperm DNA fragmentation, mitochondrial function, chromatin condensation, and apoptosis.
ABSTRACT The aim of this study was to evaluate conventional semen parameters (density, morphology, and progressive motility) and the flow-cytometric parameters of DNA fragmentation, mitochondrial membrane potential, phosphatidylserine externalization, and chromatin compactness in patients with varicocele before and after varicocelectomy. Thirty men (26.5 ± 3.2 years old, range 20-32 years) with oligoasthenoteratozoospermia and grade 3 left varicocele were selected (without other causes of male infertility). Each of them underwent sperm analysis and flow cytometric evaluation before and 4 months after subinguinal microsurgical varicocelectomy (SMV). After varicocelectomy, men had significantly higher sperm density, progressive motility, and normal forms compared with baseline. They also had a significantly lower percentage of spermatozoa with low mitochondrial membrane potential. After SMV, they showed a significantly lower percentage of spermatozoa with phosphatidylserine externalization, an early sign of apoptosis. Significantly decreased percentages of spermatozoa with abnormal chromatin compactness and spermatozoa with DNA fragmentation were found after SMV compared with baseline. Subinguinal microsurgical varicocelectomy improves sperm function in oligoasthenoteratozoospermia secondary to grade 3 left varicocele. Improvements are seen in conventional parameters and biofunctional parameters not routinely evaluated.
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ABSTRACT: OBJECTIVE: To examine clinical and laboratory variables to determine which, if any, predict improved semen parameters and pregnancy after varicocelectomy. METHODS: Surgical logs were reviewed for men who underwent varicocele ligation for a fertility concern. Clinical, demographic, and laboratory data was obtained through medical chart review and paired with postoperative reproductive outcomes obtained through chart review and survey. RESULTS: Eighty-three subjects met inclusion criteria of which 63 and 24 completed both preoperative and postoperative basic and advanced semen testing, respectively. There was a statistically significant improvement in semen concentration, total motile count, sperm DNA fragmentation index (DFI), and total normal sperm count (TNSC) after varicocelectomy. Motility improved significantly in patients with preoperative asthenospermia. Concentration and motility demonstrated mean increases of 5.2 M/mL and 18%, respectively. DFI decreased from a preoperative mean of 40.8% to a postoperative mean of 24.5%. Fifty-one percent of couples were able to conceive using natural conception, intrauterine insemination, or in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI). Pregnancy, regardless of the method used to conceive, was associated with a higher postoperative motility. The mean postoperative DFI in couples who reported a spontaneous pregnancy was 34%, which was statistically higher than the mean DFI of 17.5% in couples who reported a pregnancy with IVF/ICSI (P = .04). CONCLUSION: Varicocele ligation improves multiple semen parameters. An increase in motility was the only variable associated with postoperative pregnancy irrespective of the method by which pregnancy was obtained. Mean DFI in couples able to achieve spontaneous pregnancy was above 30% and statistically higher than couples who reported pregnancy via IVF/ICSI.Urology 02/2013; · 2.42 Impact Factor
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ABSTRACT: To analyse the levels of an indirect marker of ROS-induced lipid peroxidation [i.e. malondialdehyde (MDA)] in both testes and the levels of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase inhibitor-1 (TIMP-1) in the left testis after induction of varicocele and investigated the impact of micronised purified flavonoid fraction (MPFF) on these markers. Forty-nine adolescent (6-week-old) male Wistar rats were included in this study. The rats were divided into seven groups as follows:Group-1, control; Group-2, sham; Group-3, left varicocele-induced; Group-4, varicocele + varicocelectomy + MPFF-treated (for 4 weeks); Group-5, varicocele + MPFF-treated (for 8 weeks); Group-6, varicocele-induced and 4 weeks later, MPFF-treated (for 4 weeks); and Group-7, varicocele + varicocelectomy. MDA was measured in the tissues of both testes using the thiobarbituric acid reactivity method. The ELISA method was used for the quantification of MMP-2, MMP-9 and TIMP-1 in the left testicular tissue. The levels of MDA were significantly higher in the varicocele group than in the other groups. The MDA levels in the left testicular tissues of Group-7 were significantly higher than those of Group 4 (P = 0.03). In the varicocele group, the MMP-2 and MMP-9 levels decreased, whereas the levels of TIMP-1 increased. The tissue levels of MMP-2 in Groups 4, 5 and 7 were significantly higher than those in Group 1 (P < 0.05).Andrologia 04/2013; · 1.55 Impact Factor
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ABSTRACT: The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFkappaB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.Cancer Cell International 01/2014; 14(1):1. · 2.09 Impact Factor