Article

Unusual genotype of a Uropathogenic Escherichia coli strain assigned to the B2 phylogenetic group.

Center of Pharmaceutical Sciences, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.
Journal of clinical microbiology (Impact Factor: 4.16). 06/2011; 49(8):3105-6. DOI: 10.1128/JCM.00585-11
Source: PubMed

ABSTRACT Extraintestinal pathogenic Escherichia coli (ExPEC) cause infections such as urinary tract infections, septicemia and meningitis.…

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    ABSTRACT: Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data.
    Journal of clinical microbiology 07/2012; 50(9):3108-10. · 4.16 Impact Factor
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    ABSTRACT: The four major phylogenetic groups of Escherichia coli (A, B1, B2, D) can be determined using a multiplex PCR targeting three DNA fragments (ChuA, YjaA, TspE4.C2) published by Clermont et al. (AEM 2000, 66:4555). Isolates are assigned to one of the phylogroups based on the presence or absence of these three fragments, i.e. B1 isolates are defined by the absence of ChuA and the presence of TspE4.C2. Recently, Mendonca et al. (JCM 2011, 49:3105) proposed that an extraintestinal pathogenic E. coli positive for YjaA and TspE4.C2 was a new pathogenic genotype that should be assigned to phylogroup B2. We investigated the occurrence of this genotype among 1355 E. coli collected in Denmark from animals, meat, healthy humans, and patients. A total of four isolates were identified from pig, broiler chicken meat and patients. Investigations of multilocus sequence types, virulence genes and in vivo virulence indicated pathogenicity. Although B2 isolates are often pathogenic, some remain non-pathogenic. Until further investigations of larger collections of this genotype we do not find the evidence compelling to assign the YjaA/TspE4.C2 genotype to the B2 phylogroup.
    Journal of Medical Microbiology 05/2013; · 2.30 Impact Factor

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