A new interpretation of stomatogenesis in a peritrich ciliate: Using Campanella umbellaria as a model system

Hangzhou Key Laboratory for Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, China.
Journal of Morphology (Impact Factor: 1.74). 08/2011; 272(8):987-1006. DOI: 10.1002/jmor.10965
Source: PubMed


The process of stomatogenesis in peritrich ciliates is still incompletely understood. Previous studies on the stomatogenesis of four species of peritrichs, Telotrochidium sp., Carchesium polypinum, Opercularia coarctata, and Astylozoon pyriforme conflict with one another in some cases and omit details of events in others. We described the entire process of stomatogenesis in the peritrich ciliate Campanella umbellaria (C. umbellaria) using an improved method of staining with protargol. Our results disagree with some previous studies with regard to the formation of some rudimentary structures, reorganization of the parental haplokinety, formation of new germinal rows, and separation of daughter oral complexes. The pattern of stomatogenesis characteristic of peritrichs is compared to the stomatogenetic patterns of three other oligohymenophorean subclasses and a hypothesis about the evolution of stomatogenesis in the class Oligohymenophorea is offered. Details of stomatogenesis need to be described and verified in a greater variety of peritrichs to clarify possible differences between taxa and make it possible to relate stomatogenesis to evolution within the subclass Peritrichia. Ultrastructural studies are the next step in description of morphogenetic processes in peritrichs, and characteristics of C. umbellaria make it a useful model for this work.

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    • "In addition to the traditional characters observed in vivo, e.g. the size and shape of the zooid, the number and position(s) of the contractile vacuole(s) and the shape of the macronucleus, diagnostic characters now also include the pattern of pellicular silverlines, the number of silverlines between the scopula and the trochal band and also between the trochal band and the peristome , and the pattern of the infundibular polykineties (Clamp 1991; Foissner et al. 1992). Consequently, many new peritrich species have been described, and poorly known species redescribed, in great detail in the past decade based on these new standards (Ji and Song 2004; Pepper et al. 2013; Shi et al. 2014; Song 1997; Sun et al. 2011; Wang et al. 2011, 2012). Nevertheless, much taxonomic confusion remains, at least in part, due to the ~200- yr legacy of insufficiently described taxa. "
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