Article

Ten polymorphic microsatellite markers in Michelia maudiae (Magnoliaceae).

Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China.
American Journal of Botany (impact factor: 2.66). 12/2010; 97(12):e157-8. DOI:10.3732/ajb.1000332 pp.e157-8
Source: PubMed

ABSTRACT • Premise of the study: Michelia maudiae is a threatened species in the Magnoliaceae. Microsatellite markers were developed and characterized in M. maudiae for further investigation of its conservation genetics. • Methods and Results: Microsatellite markers were developed in M. maudiae using the Fast Isolation by AFLP of Sequences Containing Repeats protocol. Ten polymorphic microsatellites were assessed in two populations of M. maudiae. The number of alleles detected per locus varied from 1 to 8. Observed and expected heterozygosities ranged from 0.000 to 0.792 and from 0.000 to 0.826, respectively. Six primer pairs showed transferability in the two related species Michelia foveolata and Michelia chapensis. • Conclusions: This set of microsatellite markers provides a useful tool for future studies of the conservation genetics of M. maudiae and other congeneric species.

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Keywords

conservation genetics
 
Fast Isolation
 
heterozygosities
 
locus varied
 
M. maudiae
 
Magnoliaceae
 
Michelia chapensis
 
Michelia maudiae
 
Microsatellite markers
 
polymorphic microsatellites
 
primer pairs
 
Sequences Containing Repeats protocol
 
species Michelia foveolata
 
useful tool
 
• Conclusions
 
• Methods
 
• Premise
 

Ye Sun