Ten polymorphic microsatellite markers in Michelia maudiae (Magnoliaceae).
ABSTRACT • Premise of the study: Michelia maudiae is a threatened species in the Magnoliaceae. Microsatellite markers were developed and characterized in M. maudiae for further investigation of its conservation genetics. • Methods and Results: Microsatellite markers were developed in M. maudiae using the Fast Isolation by AFLP of Sequences Containing Repeats protocol. Ten polymorphic microsatellites were assessed in two populations of M. maudiae. The number of alleles detected per locus varied from 1 to 8. Observed and expected heterozygosities ranged from 0.000 to 0.792 and from 0.000 to 0.826, respectively. Six primer pairs showed transferability in the two related species Michelia foveolata and Michelia chapensis. • Conclusions: This set of microsatellite markers provides a useful tool for future studies of the conservation genetics of M. maudiae and other congeneric species.