Article

Development of SSR markers from Citrus clementina (Rutaceae) BAC end sequences and interspecific transferability in Citrus.

Centro de Protección Vegetal y Biotecnología, IVIA, Apartado Oficial 46113 Moncada (Valencia), Spain.
American Journal of Botany (Impact Factor: 2.46). 11/2010; 97(11):e124-9. DOI: 10.3732/ajb.1000280
Source: PubMed

ABSTRACT • Premise of the study: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. • Methods and Results: Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. • Conclusions: These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps.

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    • "The discrimination of somatic clones based on morphological traits can be very difficult for the citrus sharing high level of genetic similarity (Luro et al. 1995; Fang and Roose 1997). A number of studies have been performed using various molecular markers, such as RFLP, RAPD, SCAR, AFLP, SSR, ISSR, and SNPs, or cpDNA sequencing, in order to evaluate the level of genetic variability in Citrus (Luro et al. 1995; Herrero et al. 1996; Fang and Roose 1997; Federici et al. 1998; Nicolosi et al. 2000; Moore 2001; Barkley et al. 2006; Novelli et al. 2006; Luro et al. 2008; Ollitrault et al. 2010; Garcia–Lor et al. 2012). Among those, SNP markers gained popularity due to their widespread nature and potential direct link to genes responsible for the traits of agronomical importance. "
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