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Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode

Toxinology Laboratory, Oswaldo Cruz Institute (IOC), Fiocruz, Rio de Janeiro, Brazil.
Journal of proteomics (Impact Factor: 3.93). 05/2011; 74(9):1545-59. DOI: 10.1016/j.jprot.2011.04.031
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ABSTRACT Angiostrongylus costaricensis is a nematode helminth that causes an intestinal acute inflammatory process known as abdominal angiostrongyliasis, which is a poorly understood human disease occurring in Latin America. Our aim was to study the proteomic profiles of adult parasites focusing on immunogenic proteins. Total cellular extracts from both genders showed similar 2-DE profiles, with 60% of all protein spots focused between pH 5-7 and presenting molecular masses from 20.1 to 66 kDa. A total of 53 different dominant proteins were identified in our dataset and were mainly associated with the following over-represented Gene Ontology Biological Process terms: "macromolecule metabolic process", "developmental process", "response to stress", and "biological regulation". Female and male immunoblots showed similar patterns of reactive proteins. Immunoreactive spots identified by MALDI-PSD were found to represent heat shock proteins, a putative abnormal DAuer Formation family member, and galectins. To date, very few biochemical analyses have focused on the nematode Angiostrongylus costaricensis. As such, our results contribute to a better understanding of its biology and the mechanisms underlying the host-parasite relationship associated with this species. Moreover, our findings represent a first step in the search for candidate proteins for diagnostic assays and the treatment of this parasitic infection.

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Available from: Karina Rebello, Feb 24, 2014
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    • "In addition, this antigen was recently identified in Angiostrongylus costaricensis by Rebello et al. (2011) and in Angiostrongylus cantonensis by Morassutti et al. (2012). In the latter study, the authors also described peptide molecules with high homology to As37 in Baylisascaris schroederi and B. malayi. "
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    International Journal for Parasitology: Parasites and Wildlife 08/2014; 3(2). DOI:10.1016/j.ijppaw.2014.03.003
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    • "Proteomics has been producing data on a large scale; however due to the scarcity of helminth genomes, there is a need for the production of more data on ''helminth-derived'' substances. This is especially true when considering the large number of species and the plasticity of these organisms in terms of their biology, morphology , development in different hosts and infection and transmission modes (Rebello et al., 2011; Mutapi, 2012). Despite this biological variety, the murines are the current animal models of choice in the biological sciences; however, other biological systems in vivo can be promising study models and also to contribute to the elucidation of the host–parasite relationship (Bolker, 2012; Sotillo et al., 2012; Robinson et al., 2013). "
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    • "The down-regulation of IDO, NEX1 annexin, and members of the TRRAP-like family observed in adult male A. cantonensis and the up-regulation observed in adult female A. cantonensis was coincidence with those published data. It may be related to the observation that female adults survived better than the male adults [47], [48]. However, these proteins were not found to be up-regulated in FL5 compared to ML5. "
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