CTL Escape Mediated by Proteasomal Destruction of an HIV-1 Cryptic Epitope

INSERM, UMR-S945, Université Pierre et Marie Curie (UPMC), Paris, France.
PLoS Pathogens (Impact Factor: 7.56). 05/2011; 7(5):e1002049. DOI: 10.1371/journal.ppat.1002049
Source: PubMed


Cytotoxic CD8+ T cells (CTLs) play a critical role in controlling viral infections. HIV-infected individuals develop CTL responses against epitopes derived from viral proteins, but also against cryptic epitopes encoded by viral alternative reading frames (ARF). We studied here the mechanisms of HIV-1 escape from CTLs targeting one such cryptic epitope, Q9VF, encoded by an HIVgag ARF and presented by HLA-B*07. Using PBMCs of HIV-infected patients, we first cloned and sequenced proviral DNA encoding for Q9VF. We identified several polymorphisms with a minority of proviruses encoding at position 5 an aspartic acid (Q9VF/5D) and a majority encoding an asparagine (Q9VF/5N). We compared the prevalence of each variant in PBMCs of HLA-B*07+ and HLA-B*07- patients. Proviruses encoding Q9VF/5D were significantly less represented in HLA-B*07+ than in HLA-B*07- patients, suggesting that Q9FV/5D encoding viruses might be under selective pressure in HLA-B*07+ individuals. We thus analyzed ex vivo CTL responses directed against Q9VF/5D and Q9VF/5N. Around 16% of HLA-B*07+ patients exhibited CTL responses targeting Q9VF epitopes. The frequency and the magnitude of CTL responses induced with Q9VF/5D or Q9VF/5N peptides were almost equal indicating a possible cross-reactivity of the same CTLs on the two peptides. We then dissected the cellular mechanisms involved in the presentation of Q9VF variants. As expected, cells infected with HIV strains encoding for Q9VF/5D were recognized by Q9VF/5D-specific CTLs. In contrast, Q9VF/5N-encoding strains were neither recognized by Q9VF/5N- nor by Q9VF/5D-specific CTLs. Using in vitro proteasomal digestions and MS/MS analysis, we demonstrate that the 5N variation introduces a strong proteasomal cleavage site within the epitope, leading to a dramatic reduction of Q9VF epitope production. Our results strongly suggest that HIV-1 escapes CTL surveillance by introducing mutations leading to HIV ARF-epitope destruction by proteasomes.

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Available from: Peter-Michael Kloetzel, Oct 13, 2015
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    • "It is possible that this intra-epitope mutation impairs processing of the MHC-bound 9-mer, which would not be evidenced by recognition of peptides but would require introduction of the mutation into the virus and measurement of recognition of infected cells. This situation was described for a cryptic epitope in HIV-1 [17]. As mentioned above, acute phase PBMC were no longer available from animal r04028 that could be used to compare recognition of the wild type AF8 peptide with the mutated peptide harboring a position 8 F to S mutation (the intra-epitope mutation detected at time of euthanasia). "
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