Article

Foot and mouth disease virus polyepitope protein produced in bacteria and plants induces protective immunity in guinea pigs.

Bioengineering Center, Russian Academy of Sciences, Moscow, Russia.
Biochemistry (Moscow) (impact factor: 1.06). 03/2011; 76(3):339-46. pp.339-46
Source: PubMed

ABSTRACT The goal of this project was to develop an alternative foot and mouth disease (FMD) vaccine candidate based on a recombinant protein consisting of efficient viral epitopes. A recombinant gene was designed that encodes B-cell epitopes of proteins VP1 and VP4 and T-cell epitopes of proteins 2C and 3D. The polyepitope protein (H-PE) was produced in E. coli bacteria or in N. benthamiana plants using a phytovirus expression system. The methods of extraction and purification of H-PE proteins from bacteria and plants were developed. Immunization of guinea pigs with the purified H-PE proteins induced an efficient immune response against foot and mouth disease virus (FMDV) serotype O/Taiwan/99 and protection against the disease. The polyepitope protein H-PE can be used as a basis for developing a new recombinant vaccine against FMD.

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    ABSTRACT: Advances in transient expression technologies have allowed the production of milligram quantities of proteins within a matter of days using only small amounts (tens of grams) of plant tissue. Among the proteins that have been produced using this approach are the structural proteins of viruses which are capable of forming virus-like particles (VLPs). As such particulate structures are potent stimula-tors of the immune system, they are excellent vaccine candidates both in their own right and as carriers of additional immunogenic se-quences. VLPs of varying complexity derived from a variety of animal viruses have been successfully transiently expressed in plants and their immunological properties assessed. Generally, the plant-produced VLPs were found to have the expected antigenicity and immuno-genicity. In several cases, including an M2e-based influenza vaccine candidate, the plant-expressed VLPs have been shown to be capable of stimulating protective immunity. These findings raise the prospect that low-cost plant-produced vaccines could be developed for both veterinary and human use.-like particle, hepatitis B core antigen, human papillomavirus, bovine papillomavirus, bluetongue vi-rus, foot-and-mouth disease virus, influenza virus, porcine respiratory and reproductive syndrome virus, protective immunity.
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

alternative foot
 
E. coli bacteria
 
efficient immune response
 
efficient viral epitopes
 
encodes B-cell epitopes
 
guinea pigs
 
H-PE
 
H-PE proteins
 
mouth disease
 
mouth disease virus
 
N. benthamiana plants
 
new recombinant vaccine
 
phytovirus expression system
 
polyepitope protein
 
polyepitope protein H-PE
 
proteins 2C
 
proteins VP1
 
purified H-PE proteins induced
 
recombinant protein