L1TD1 Is a Marker for Undifferentiated Human Embryonic Stem Cells

Department of Biological Chemistry, University of California Irvine, Irvine, California, United States of America.
PLoS ONE (Impact Factor: 3.53). 04/2011; 6(4):e19355. DOI: 10.1371/journal.pone.0019355
Source: PubMed

ABSTRACT Human embryonic stem cells (hESC) are stem cells capable of differentiating into cells representative of the three primary embryonic germ layers. There has been considerable interest in understanding the mechanisms regulating stem cell pluripotency, which will ultimately lead to development of more efficient methods to derive and culture hESC. In particular, Oct4, Sox2 and Nanog are transcription factors known to be important in maintenance of hESC. However, many of the downstream targets of these transcription factors are not well characterized. Furthermore, it remains unknown whether additional novel stem cell factors are involved in the establishment and maintenance of the stem cell state.
Here we show that a novel gene, L1TD1 (also known as FLJ10884 or ECAT11), is abundantly expressed in undifferentiated hESC. Differentiation of hESC via embryoid body (EB) formation or BMP4 treatment results in the rapid down-regulation of L1TD1 expression. Furthermore, populations of undifferentiated and differentiated hESC were sorted using the stem cell markers SSEA4 and TRA160. Our results show that L1TD1 is enriched in the SSEA4-positive or TRA160-positive population of hESC. Using chromatin immunoprecipitation we found enriched association of Nanog to the predicted promoter region of L1TD1. Furthermore, siRNA-mediated knockdown of Nanog in hESC also resulted in downregulation of L1TD1 expression. Finally, using luciferase reporter assay we demonstrated that Nanog can activate the L1TD1 upstream promoter region. Altogether, these results provide evidence that L1TD1 is a downstream target of Nanog.
Taken together, our results suggest that L1TD1 is a downstream target of Nanog and represents a useful marker for identifying undifferentiated hESC.

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Available from: Peter J Donovan, Dec 19, 2013
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    • "The role of post-transcriptional regulators and protein networks in the maintenance of pluripotency is still largely unknown, especially in human cells. L1TD1 is highly and specifically expressed in pluripotent cells (Iwabuchi et al., 2011; Mitsui et al., 2003; Närvä et al., 2012; Wong et al., 2011) under the control of OCT4, NANOG, and SOX2 (Boyer et al., 2005; Närvä et al., 2012; Wong et al., 2011). We previously reported that L1TD1 is essential for maintaining the pluripotent state in hESCs (Närvä et al., 2012). "
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    ABSTRACT: The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
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