Article

Membrane array analysis of tear proteins in ocular cicatricial pemphigoid.

Francis I. Proctor Research Foundation, University of California, San Francisco Medical School, San Francisco, California, USA.
Optometry and vision science: official publication of the American Academy of Optometry (impact factor: 1.53). 05/2011; 88(8):1005-9. DOI:10.1097/OPX.0b013e31821ddc6c pp.1005-9
Source: PubMed

ABSTRACT To explore non-invasive, protein-based, membrane array technology as a means to evaluate the global immune and angiogenic profile of tear proteins in patients with active ocular cicatricial pemphigoid (OCP).
Forty-three proteins consisting of cytokines, angiogenic/growth factors, and immunoinflammatory modulators were measured by membrane array in tear samples of four control patients and four OCP patients during active disease and after treatment.
Signals for several distinct and consistent molecular entities were upregulated in all four active OCP tear samples relative to controls. In particular, interleukin-8 and matrix metalloproteinase-9 were elevated during active disease and decreased after systemic immunomodulatory therapy.
Protein array analysis may provide a well-tolerated assay to monitor levels of inflammatory markers in the tears of OCP patients in response to therapy.

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Keywords

active ocular cicatricial pemphigoid
 
angiogenic profile
 
consistent molecular entities
 
control patients
 
cytokines
 
global immune
 
immunoinflammatory modulators
 
inflammatory markers
 
matrix metalloproteinase-9
 
membrane array
 
membrane array technology
 
OCP patients
 
patients
 
Protein array analysis
 
protein-based
 
proteins
 
Signals
 
systemic immunomodulatory therapy
 
tear proteins
 
well-tolerated assay