Article

[Directional differentiation of human adipose-derived stem cells into endothelial cells].

Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China.
Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery 01/2011; 27(1):58-62. pp.58-62
Source: PubMed

ABSTRACT To investigate the possibility of directional differentiation of human adipose stem cells (hADSCs) into endothelial cells (EC), so as to provide seed cells for tissue engineered vessels.
hADSCs were isolated from human adipose tissue by collagenase digestion, cultured and amplified by adherence to flasks. Then hADSCs were directionally induced to differentiate into EC by a combination of fibronectin (FN), endothelial cells support liquid (EGM2-MV) containing various growth factors and high concentration of VEGF165 (50 ng/ml). Then, the cells morphology, phenotype and function were identified.
Highly homologous hADSCs were obtained, and then hADSCs were directionally differentiated into EC. CD31 and CD34, the specific markers for EC, and vascular endothelial growth factor receptor (KDR) were positive by immunohistochemical staining and RT-PCR. In addition, unique Weibel-Palade bodies in EC were observed under transmission electron microscope. Functionally, hADSCs could swallow Dil-Ac-LDL and form tube-like structures in matrigel after endothelial differentiation.
hADSCs can be successfully induced to differentiate into endothelial cells in vitro.

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Keywords

adherence
 
cells morphology
 
collagenase digestion
 
Dil-Ac-LDL
 
directional differentiation
 
EC
 
endothelial cells
 
endothelial cells support liquid
 
endothelial differentiation
 
form tube-like structures
 
homologous hADSCs
 
human adipose
 
human adipose tissue
 
immunohistochemical staining
 
seed cells
 
specific markers
 
transmission electron microscope
 
unique Weibel-Palade bodies
 
various growth factors
 
vascular endothelial growth factor receptor