Article

Levels of arsenic, cadmium, lead, manganese and zinc in biological samples of paralysed steel mill workers with related to controls.

National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, 76080, Pakistan.
Biological trace element research (Impact Factor: 1.92). 05/2011; 144(1-3):164-82. DOI: 10.1007/s12011-011-9063-4
Source: PubMed

ABSTRACT The determination of essential trace and toxic elements in the biological samples of human beings is an important clinical screening procedure. This study aimed to assess the possible effects of environmental exposure on paralysed male workers (n = 75) belonging to the production and quality control departments of a steel mill. In this investigation, the concentrations of arsenic, cadmium, lead, manganese and zinc were determined in biological samples (blood, urine and scalp hair samples) of exposed paralysis and non-paralysed steel mill workers. For comparative purposes, unexposed healthy subjects of same age group were selected as referents. The elements in the biological samples were measured by atomic absorption spectrophotometry prior to microwave-assisted acid digestion. The validity of the methodology was checked by the biological certified reference materials. The results indicate that the level understudy elements in all three biological samples were significantly higher in paralysed workers of both groups (quality control and production) as compared to referents (p < 0.01). The possible connection of these elements with the aetiology of disease is discussed. The results also show the need for immediate improvements of workplace ventilation and industrial hygiene practices.

1 Bookmark
 · 
148 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study aimed to investigate the distribution and factors influencing blood levels of Cadmium (Cd), Arsenic (As), and Manganese (Mn), and to determine their reference values in a sample of blood donors residing in Rio Branco, capital city of Acre State, Brazil. Blood samples were collected from all blood donors attending the Central Hemotherapic Unit in Rio Branco between 2010 and 2011. Among these, 1183 donors (98.9%) answered to a questionnaire on sociodemographic and lifestyle factors. Blood metal concentrations were determined by atomic spectrometry. Association between Cd, As and Mn levels and donors' characteristics was examined by linear regression analysis. Reference values were estimated as the upper limit of the 95% confidence interval of the 95th percentile of metal levels. References values were 0.87μgL(-1) for Cd, 9.87μgL(-1) for As, and 29.32μgL(-1) for Mn. Reference values of Cd and As in smokers were 2.66 and 10.86μgL(-1), respectively. Factors contributing to increase Cd levels were smoking, ethnicity (non-white), and lower education, whereas drinking tea and non-bottled water were associated with lower Cd. Lower levels of As were associated with higher household income, living near industrial facilities, working in a glass factory, a compost plant or in metal mining activities. Risk factors for Mn exposure were not identified. In general, blood Cd concentrations were in the range of exposure levels reported for other people from the general population, whereas levels of As and Mn were higher than in other non-occupationally exposed populations elsewhere. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Chemosphere 02/2015; 128C:70-78. DOI:10.1016/j.chemosphere.2014.12.083 · 3.50 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Although the toxic metal Cd is an established human nephrotoxin, little is known about the role that interactions with plasma constitutents play in determining its mammalian target organs. To gain insight, a Cd-human serum albumin (HSA) complex was analyzed on a system consisting of size exclusion chromatography (SEC) coupled on-line to a flame atomic absorption spectrometer (FAAS). Using phosphate buffered saline (pH 7.4) as the mobile phase, we investigated the effect of 1-10mM oxidized glutathione (GSSG), l-cysteine (Cys), l-glutathione (GSH), or N-acetyl-l-cysteine (NAC) on the elution of Cd. As expected, GSSG did not mobilize Cd from the Cd-HSA complex up to a concentration of 4mM. With 1.0mM NAC, ∼30% of the injected Cd-HSA complex eluted as such, while the mobilized Cd was lost on the column. With 1.0mM of Cys or GSH, no parent Cd-HSA complex was detected and 88% and 82% of the protein bound Cd eluted close to the elution volume, likely in form of Cd(Cys)2 and a Cd-GSH 1:1 complex. Interestingly, with GSH and NAC concentrations >4.0mM, a Cd double peak was detected, which was rationalized in terms of the elution of a polynuclear Cd complex baseline-separated from a mononuclear Cd complex. In contrast, mobile phases which contained Cys concentrations ≥2mM resulted in the detection of only a single Cd peak, probably Cd(Cys)4. Our results establish SEC-FAAS as a viable tool to probe the mobilization of Cd from binding sites on plasma proteins at near physiological conditions. The detected complexes between Cd and Cys or GSH may be involved in the translocation of Cd to mammalian target organs.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 03/2014; 958C:16-21. DOI:10.1016/j.jchromb.2014.03.012 · 2.78 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A digestion free blood arsenic assessment method based upon in situ trapping of arsenic hydride in coated graphite furnace was developed. Double layer coating of the furnace with Na2WO4 – H2PtCl6 was used. Arsenic limit of detection (3σ) for whole blood samples was 0.1 μg/L. Dynamic range was 60-1000 pg As. Relative standard deviation for blood samples (n = 3) was 7–13%. Blood arsenic biomonitoring in infants residing in an industrially polluted area was performed using the designed method. Whole blood arsenic determination was performed in 92 subjects of case group and 56 subjects of age-matched control group. For both groups observed blood arsenic distribution was found to be close to lognormal. Lognormalized mean blood arsenic for the case was 5.89 ± 1.31 μg/L; for the controls 1.50 ± 2.26 μg/L. Significant blood arsenic elevation in the case group of the infants under study compared to the controls and previously published data was observed.
    Journal of Analytical Atomic Spectrometry 06/2014; 29(10). DOI:10.1039/C4JA00130C · 3.40 Impact Factor