Article

Biosafety assessment of Gd@C-82(OH)(22) nanoparticles on Caenorhabditis elegans

CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology, Beijing, China.
Nanoscale (Impact Factor: 6.74). 06/2011; 3(6):2636-41. DOI: 10.1039/c1nr10239g
Source: PubMed

ABSTRACT Gd@C(82)(OH)(22), a water-soluble endohedral metallofullerene derivative, has been proven to possess significant antineoplastic activity in mice. Toxicity studies of the nanoparticle have shown some evidence of low or non toxicity in mice and cell models. Here we employed Caenorhabditis elegans (C. elegans) as a model organism to further evaluate the short- and long-term toxicity of Gd@C(82)(OH)(22) and possible behavior changes under normal and stress culture conditions. With treatment of Gd@C(82)(OH)(22) at 0.01, 0.1, 1.0 and 10 μg ml(-1) within one generation (short-term), C. elegans showed no significant decrease in longevity or thermotolerance compared to the controls. Furthermore, when Gd@C(82)(OH)(22) treatment was extended up to six generations (long-term), non-toxic effects to the nematodes were found. In addition, data from body length measurement, feeding rate and egg-laying assays with short-term treatment demonstrated that the nanoparticles have no significant impact on the individual growth, feeding behavior and reproductive ability, respectively. In summary, this work has shown that Gd@C(82)(OH)(22) is tolerated well by worms and it has no apparent toxic effects on longevity, stress resistance, growth and behaviors that were observed in both adult and young worms. Our work lays the foundations for further developments of this anti-neoplastic agent for clinical applications.

0 Followers
 · 
167 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Reconstrucción documental y análisis al unísono del debate público desarrollado en torno a "la cuestión de la mujer" en Gran Bretaña y Estados Unidos durante los años 1837-1883, correspondientes al reinado de Victoria I en la monarquía británica, en el que tomaron parte tanto hombres como mujeres, principalmente de la clase media. Aunque el período victoriano es sólo un capítulo, con especificidad geográfica, en la historia en torno a esta discusión, supone una etapa con características propias, manifestadas incluso en un conjunto de términos como "la misión de la mujer", "la esfera de la mujer" o "la influencia de la mujer". En contra de la idea más o menos generalizada de que durante esta época imperó una sola actitud respecto a la condición femenina, las autoras argumentan que el estudio de la opinión pública británica y norteamericana de esos años muestra que la forma predominante de la escritura victoriana en torno a las mujeres no fue el pronunciamiento, sino el debate. Dividido en tres tomos, el primero de ellos está enfocado en textos representativos, incluido una de la propia reina Victoria, figuras y controversias que presentan un panorama general de "la cuestión de la mujer"; el segundo sigue la huella del debate en los discursos de la ley, la ciencia, el trabajo y la religión y el último está dedicado a las discusiones en torno a la figura de la mujer escritora y a una serie de heroínas literarias.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Silver nanoparticles (NPs) are used for a wide range of commercial reasons to restrict microbial growth. The increasing use of silver NPs in modern materials ensures they will find their way into environmental systems. The mode of action which makes them desirable as an antimicrobial tool could also pose a severe threat to the natural microbial balance existing in these systems. Research into the potential environmental threats of silver NPs has mainly focused on particular areas, such as their influence in rivers and estuaries or their effect on organisms such as earthworms and plants. There is a need to focus studies on all aspects of the microbial world and to highlight potential risks and methods of overcoming problems before significant damage is done. This review focuses on the antimicrobial uses, mechanisms of toxicity, and effects on the environment (mainly soil) of silver NPs, illustrating gaps in current knowledge.
    Advances in applied microbiology 01/2011; 77:115-33. DOI:10.1016/B978-0-12-387044-5.00005-4 · 2.24 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Nanoparticles (NPs) are attracting increased attention in commerce and applied microbiology due to their antimicrobial activity, high electrical conductivity, and optical properties. For example, silver NPs have broad spectrum antimicrobial properties against a wide range of bacteria and fungi, making them ideal for minimizing biofouling. By controlling the size, shape, surface, and agglomeration state of the NPs, specific ion release profiles can be developed for any given application. Currently, NPs are formed in a wide variety of different shapes and sizes including spheres, plates, and wires. This review looks at both commercially and naturally produced NPs with a focus on silver NPs and addresses how these are formed. Furthermore, potential areas for improving these techniques will be highlighted, focusing on advancing shape and structure formation using modern applications. Finally, the review evaluates the feasibility of bioengineering microorganisms to synthesize particles of defined shape and size, by examining genes associated with NP production.
    Advances in applied microbiology 01/2012; 80:113-42. DOI:10.1016/B978-0-12-394381-1.00005-2 · 2.24 Impact Factor