Article

Associated chromosome trap for identifying long-range DNA interactions.

Medical Service, VA Palo Alto Health Care System, USA.
Journal of Visualized Experiments 01/2011; DOI: 10.3791/2621
Source: PubMed

ABSTRACT Genetic information encoded by DNA is organized in a complex and highly regulated chromatin structure. Each chromosome occupies a specific territory, that may change according to stage of development or cell cycle. Gene expression can occur in specialized transcriptional factories where chromatin segments may loop out from various chromosome territories, leading to co-localization of DNA segments which may exist on different chromosomes or far apart on the same chromosome. The Associated Chromosome Trap (ACT) assay provides an effective methodology to identify these long-range DNA associations in an unbiased fashion by extending and modifying the chromosome conformation capture technique. The ACT assay makes it possible for us to investigate mechanisms of transcriptional regulation in trans, and can help explain the relationship of nuclear architecture to gene expression in normal physiology and during disease states.

0 Followers
 · 
130 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Mounting evidence is compiling linking the physical organizational structure of chromosomes and the nuclear structure to biological function. At the base of the physical organizational structure of both is the concept of loop formation. This implies that physical proximity within chromosomes is provided for otherwise distal genomic regions and thus hierarchically organizing the chromosomes. Together with entropy many experimental observations can be explained with these two concepts. Among the observations that can be explained are the measured physical extent of the chromosomes, their shape, mechanical behavior, the segregation into territories (chromosomal and territories within chromosomes), the results from chromosome conformation capture experiments, as well as linking gene expression to structural organization.
    Experimental Cell Research 04/2012; 318(12):1381-5. DOI:10.1016/j.yexcr.2012.03.027 · 3.37 Impact Factor

Preview (2 Sources)

Download
2 Downloads
Available from