Article

Experimental design applied to the analysis of volatile compounds in apple juice by headspace solid-phase microextraction.

Industrial Química del Nalón S.A., Oviedo, Spain.
Journal of Separation Science (impact factor: 2.73). 06/2011; 34(11):1293-8. DOI:10.1002/jssc.201000881 pp.1293-8
Source: PubMed

ABSTRACT A simple and fast method based on solid-phase microextraction (SPME) followed by fast gas chromatography (Fast GC) has been developed for the analysis of volatile compounds in Asturian apple juices employed in the cider production. Three different fiber coatings have been checked (PDMS, PDMS-DVB and CAR-PDMS) and PDMS-DVB has been presented to be the most suitable one. Experimental design has been employed in the optimization of extraction factors and robustness assessment. The use of Fast GC allowed the separation of 14 compounds (esters, aldehydes and alcohols) in approximately 4 min, clearly reducing the analysis time when compared to conventional GC. Good linearity, recoveries and repeatability of the solid-phase microextraction were obtained with r(2) values, recoveries and relative standard deviations ranging from 0.9822 to 0.9998, 83.2 to 109.8% and 0.5 to 11.7%, respectively, using standard solution.

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    Article: Analysis of Volatile Compounds from Solanum betaceum Cav. Fruits from Panama by Head-Space Micro Extraction
    Armando A Durant, Candelario Rodríguez, Ana I Santana, Carlos Herrero, Juan C Rodríguez, Mahabir P Gupta
    [show abstract] [hide abstract]
    ABSTRACT: The characterization of the volatile compounds of two varieties of Solanum betaceum Cav. by means of headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) is presented. The HS-SPME method for extraction of the volatiles compounds was optimized by using a 2 3 central composite design. Maximum extraction of volatile compounds was achieved by using a divinylbenzene-carboxen-polydimethylsiloxane (DVB/CAR/PDMS) fiber, extraction temperature 76° C, incubation time 44 min, and extraction time of 46 min. The main types of compounds detected in both varieties are terpenoids, followed by aromatics, esters, and aldehydes. Golden-yellow cultivars contained higher levels of esters and terpenes, while the reddish-purple variety contained a significant amount of aromatic compounds. The data structure of the chemical information obtained as well as the relationship between variables was evaluated by means of principal component analysis and cluster analysis.
    Records of Natural Products 01/2013; 7:15-26. · 1.51 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

14 compounds
 
4 min
 
alcohols
 
analysis time
 
Asturian apple juices
 
conventional GC
 
different fiber coatings
 
extraction factors
 
fast gas chromatography
 
Fast GC
 
fast method
 
Good linearity
 
recoveries
 
relative standard deviations
 
solid-phase microextraction
 
SPME