Prevalence and Characterization of Campylobacter jejuni Isolated from Pasture Flock Poultry

Dept. of Food Science, Center for Food Safety-Inst. of Food Science & Engineering, Univ. of Arkansas, Fayetteville, AR 72704, USA.
Journal of Food Science (Impact Factor: 1.7). 09/2010; 75(7):M496-502. DOI: 10.1111/j.1750-3841.2010.01747.x
Source: PubMed

ABSTRACT The growing interest in organic and natural foods warrants a greater need for information on the food safety of these products. In this study, samples were taken from 2 pasture flock farms (N= 178; feed, water, drag swabs, and insect traps), pasture flock retail carcasses (N= 48) and 1 pasture flock processing facility (N= 16) over a period of 8 mo. A total of 105 Campylobacter isolates were obtained from 53 (30%), 36 (75%), and 16 (100%) samples from the farms, retail carcasses, and processing facility, respectively. Of the 105 isolates collected, 65 were C. jejuni, 31 were C. coli, and 9 were other Campylobacter spp. Using PCR, the C. jejuni isolates were further analyzed for virulence genes involved in colonization and survival (flaA, flaC, cadF, dnaJ, racR, cbrR), invasion (virB11, ciaB, pldA), protection against harsh conditions (sodB, htrA, clpA), toxin production (cdtA, cdtB, cdtC), siderophore transport (ceuE), and ganglioside mimicry (wlaN). In addition, the short variable region of the flaA locus (flaA SVR) was sequenced to determine the genetic diversity of the C. jejuni isolates. The flaA SVR diversity indices increased along the farm to carcass continuum. PCR-based analysis indicated a low prevalence of 5 genes involved in colonization (dnaJ, ciaB, pldA, racR, virB11). The results of this survey indicate that the prevalence of Campylobacter on organic retail carcasses is similar to prevalence reports of Campylobacter on conventional retail carcasses. However, the genetic diversity of the flaA SVR genotypes increased along the farm to carcass continuum that contrasted with conventional poultry studies. 2010 Institute of Food Technologists®.

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    • "In MCLF or backyard farming practice, postharvest processing can be carried out manually on the farm in rudimentary facilities. As a result, prevalence of foodborne pathogens is highly variable from farm to farm or even day to day (Melendez et al., 2010; Hanning et al., 2010; Salaheen and Biswas, unpublished data). MCLFs or backyard farmers ideally and conceptually follow similar poultry processing techniques that are adopted in large processing plants, but on a much smaller scale. "
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    ABSTRACT: Use of mixed crop-livestock farms (MCLFs) is one of the oldest and most traditional farming methods practiced all over the world, and MCLFs are still one of the major systems of food production, particularly for organic foods. On these typically small farms, livestock are reared primarily on grass and naturally grown crops, while composted animal wastes are used to fertilize the soil for growing crops. Specific to organic MCLFs, biosecurity challenges arise from the fact that animals are reared outdoors, which increases potential for contact with disease vectors including wild birds, rodents, and insects. Organic regulations do not allow the use of chemicals and antibiotics; therefore, alternative methods for control of disease and zoonotic pathogens must be used. Due to the biosecurity challenges and the complexity of the MCLF environment, methods for control of zoonotic pathogens need to be carefully considered in order to be effective and to abide by organic regulations if required. The objectives of this study are to define the complex routes of transmission, as well as the prevalence of potential zoonotic and possible interruption strategies of these pathogens among the food animals and crops produced on MCLFs. © 2015 Poultry Science Association Inc.
    Poultry Science 01/2015; DOI:10.3382/ps/peu055 · 1.67 Impact Factor
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    • "Furthermore, most of these studies have detected similarities in Campylobacter prevalence between conventional and organic flocks, indicating that environmental conditions have minimal influence on overall Campylobacter contamination levels (Cui et al., 2005; Han et al., 2009). Hanning et al. (2010) screened 242 samples from 2 pasture flocks, facilities, and retail carcasses for 8 mo and isolated 105 Campylobacter species (43%). Han et al. (2009) also detected a 43.3% Campylobacter contamination level in birds raised in conventional and organic chickens in Louisiana. "
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