[Show abstract][Hide abstract] ABSTRACT: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase
activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early
lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental
potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage,
bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium
(ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.
[Show abstract][Hide abstract] ABSTRACT: Chemically defined medium (CDM) conditions for controlling human embryonic stem cell (hESC) fate will not only facilitate the practical application of hESCs in research and therapy but also provide an excellent system for studying the molecular mechanisms underlying self-renewal and differentiation, without the multiple unknown and variable factors associated with feeder cells and serum. Here we report a simple CDM that supports efficient self-renewal of hESCs grown on a Matrigel-coated surface over multiple passages. Expanded hESCs under such conditions maintain expression of multiple hESC-specific markers, retain the characteristic hESC morphology, possess a normal karyotype in vitro, as well as develop teratomas in vivo. Additionally, several growth factors were found to selectively induce monolayer differentiation of hESC cultures toward neural, definitive endoderm/pancreatic and early cardiac muscle cells, respectively, in our CDM conditions. Therefore, this CDM condition provides a basic platform for further characterization of hESC self-renewal and directed differentiation, as well as the development of novel therapies.
Proceedings of the National Academy of Sciences 06/2006; 103(18):6907-12. DOI:10.1073/pnas.0602280103 · 9.67 Impact Factor
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