Two new anti-aging compounds, ganodermasides C and D, were isolated and their structures elucidated. They are novel ergosterols possessing a 4,6,8(14),22-tetraene-3-one unit with unique hydroxylation at C-9. Both of them significantly extended the replicative lifespan of the K6001 yeast strain. Ganodermasides C and D regulated the expression of the gene for UTH1 to prolong the replicative lifespan of yeast.
[Show abstract][Hide abstract] ABSTRACT: Objective
Extracts of Ganoderma species have been widely used as herbal medicines in the treatment of several infections. This study was carried out to ascertain the haematological properties of aqueous Ganoderma applanatum (G. applanatum).Methods
Sixty albino rats grouped into six equal groups (10 each) of A to F consisting of tests and controls. Laboratory albino rats in groups A, B and C were infected with Trypanosoma brucei brucei (T. brucei brucei) while groups A and B (test) were treated with aqueous G. applanatum extract; other groups served as control. Microscopy and haematological profiles from the albino rats were monitored on daily basis for blood parasites, packed cell volume (PCV), haemoglobin concentration (HC), total red blood cell count (RBC), mean cell haemoglobin (MCH), mean cell volume (MCV), mean cell haemoglobin concentration (MCHC), and total white blood cell count (WBC).ResultsAlbino rats in groups A, B and C infected with T. brucei brucei and treated with various concentrations of aqueous G. applanatum showed a progressive reduction in PCV, HC, RBC, MCH and MCHC compared to the controls (P<0.05). All the infected rats died by day 14 of the experiment from parasitaemia.ConclusionsG. applanatum lacks ability to boost haematological profiles of anaemic laboratory rats and also of no use in the treatment of African trypanosomiasis. Higher doses of the fungal extract may be required to test on laboratory rats with less lethal biological stimulants of anaemia before proving or otherwise its true haematological properties.
Asian Pacific Journal of Tropical Disease 10/2012; 2(5):390–394. DOI:10.1016/S2222-1808(12)60085-1
[Show abstract][Hide abstract] ABSTRACT: Nolinospiroside F is a steroidal saponin isolated from Ophiopogon japonicus (O. japonicus). In this study, we found that nolinospiroside F significantly extends the replicative lifespan of K6001 yeast at doses of 1, 3 and 10 μM, indicating that it has an anti-aging effect. This may be attributed to its anti-oxidative effect, as nolinospiroside F could increase yeast survival under oxidative stress conditions and decrease the level of malondialdehyde (MDA), an oxidative stress biomarker. It could also increase anti-oxidative stress genes, SOD1 and SOD2, expression, and the activity of superoxide dismutase (SOD). It increase the activity of SIRT1, an upstream inducer of SOD2 expression. In sod1 and sod2 mutant yeast strains, nolinospiroside F failed to extend their replicative lifespan. These results indicate that SOD participates in the anti-aging effect of nolinospiroside F. Furthermore, nolinospiroside F inhibited the expression of UTH1, a yeast-aging gene that is involved in the oxidative stress of yeast, and failed to extend the replicative lifespan of uth1 or skn7 mutant yeast cells. SKN7 is the transcriptional activator of UTH1. We also demonstrate that SOD and UTH1 regulate each other's expression. Together, these results suggest that SOD and UTH1 genes are required for and play interactive roles in nolinospiroside F-mediated yeast lifespan extension.
International Journal of Molecular Sciences 03/2013; 14(3):4461-75. DOI:10.3390/ijms14034461 · 2.86 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Two new steroids, (14β,22E)-9,14-dihydroxyergosta-4,7,22-triene-3,6-dione (1) and (5α,6β,15β,22E)-6-ethoxy-5,15-dihydroxyergosta-7,22-dien-3-one (2), together with three known steroids, calvasterols A and B (3 and 4, resp.), and ganodermaside D (5), were isolated from the culture broth of an endophytic fungus Phomopsis sp. isolated from Aconitum carmichaeli. The structures of these compounds were elucidated on the basis of spectroscopic analysis, and their inhibitory activities against six pathogenic fungi were evaluated. Most of the compounds showed moderate or weak antifungal activities in a broth-microdilution assay.
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.