Article

Controlled growth and differentiation of MSCs on grooved films assembled from monodisperse biological nanofibers with genetically tunable surface chemistries.

Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, OK 73019, USA.
Biomaterials (impact factor: 7.4). 07/2011; 32(21):4744-52. DOI:10.1016/j.biomaterials.2011.03.030 pp.4744-52
Source: PubMed

ABSTRACT The search for a cell-supporting scaffold with controlled topography and surface chemistry is a constant topic within tissue engineering. Here we have employed M13 phages, which are genetically modifiable biological nanofibers (∼ 880 nm long and ∼ 6.6 nm wide) non-toxic to human beings, to form films for supporting the growth of mesencymal stem cells (MSCs). Films were built from nearly parallel phage bundles separated by grooves. The bundles can guide the elongation and alignment of MSCs along themselves. Phage with peptides displayed on the surface exhibited different control over the fine morphologies and differentiation of the MSCs. When an osteogenic peptide was displayed on the surface of phage, the proliferation and differentiation of MSCs into osteoblasts were significantly accelerated. The use of the grooved phage films allows us to control the proliferation and differentiation of MSCs by simply controlling the concentrations of phages as well as the peptides displayed on the surface of the phages. This work will advance our understanding on the interaction between stem cells and proteins.

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Keywords

alignment
 
cell-supporting scaffold
 
constant topic
 
elongation
 
fine morphologies
 
form films
 
genetically modifiable biological nanofibers
 
grooved phage films
 
grooves
 
M13 phages
 
osteoblasts
 
osteogenic peptide
 
parallel phage bundles
 
peptides
 
phage
 
phages
 
surface chemistry
 
surface exhibited different control
 
tissue engineering
 
topography
 

Haibao Zhu