The effect of H. pylori eradication on meal-associated changes in plasma ghrelin and leptin

New York University Langone Medical Center, New York, NY, USA.
BMC Gastroenterology (Impact Factor: 2.11). 04/2011; 11:37. DOI: 10.1186/1471-230X-11-37
Source: PubMed

ABSTRACT Appetite and energy expenditure are regulated in part by ghrelin and leptin produced in the gastric mucosa, which may be modified by H. pylori colonization. We prospectively evaluated the effect of H. pylori eradication on meal-associated changes in serum ghrelin and leptin levels, and body weight.
Veterans referred for upper GI endoscopy were evaluated at baseline and ≥8 weeks after endoscopy, and H. pylori status and body weight were ascertained. During the first visit in all subjects, and during subsequent visits in the initially H. pylori-positive subjects and controls, blood was collected after an overnight fast and 1 h after a standard high protein meal, and levels of eight hormones determined.
Of 92 enrolled subjects, 38 were H. pylori-negative, 44 H. pylori-positive, and 10 were indeterminate. Among 23 H. pylori-positive subjects who completed evaluation after treatment, 21 were eradicated, and 2 failed eradication. After a median of seven months following eradication, six hormones related to energy homeostasis showed no significant differences, but post-prandial acylated ghrelin levels were nearly six-fold higher than pre-eradication (p=0.005), and median integrated leptin levels also increased (20%) significantly (p<0.001). BMI significantly increased (5 ± 2%; p=0.008) over 18 months in the initially H. pylori-positive individuals, but was not significantly changed in those who were H. pylori-negative or indeterminant at baseline.
Circulating meal-associated leptin and ghrelin levels and BMI changed significantly after H. pylori eradication, providing direct evidence that H. pylori colonization is involved in ghrelin and leptin regulation, with consequent effects on body morphometry.


Available from: Joshua R. Shak, Jun 15, 2015
1 Follower
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: Take in to account the relationship between obesity and many diseases and contradictory published results considering the effects of H. pylori infection on leptin and ghrelin levels, we decided to determine the effect of H. pylori eradication on body composition, dietary intake, leptin and ghrelin levels of infected patients. Methods: This study included 100 patients. After endoscopy, active infection with H. pylori was determined by rapid urease test and histopathology evaluation. Eradication was confirmed by the urea breath test at 3 months. The body weight, body composition and dietary intake of patients were assessed by Seca scale, Maltron Bioscan 916 and 24-hour recall food questionnaire respectively before and after eradication. Serum leptin and ghrelin were determined by enzyme linked immunosorbent assay (ELISA) kits. Results: The mean body weight, fat mass and body cell mass of patients increased after eradication but only the changes of body weight was statistically significant (P=0.01). The mean free fat mass and percentage of free fat mass decreased significantly at the end of study (P<0.05). Eradication has no significant effect on dietary intake, serum leptin and ghrelin levels. Conclusion: According to our findings, eradication of H. pylori lead to a statistically significant increase of body weight and fat mass in patients while dietary intake, serum leptin and ghrelin levels of subjects did not change after treatment. It seems that enhanced incidence of gastro-esophageal reflux disease after H. pylori eradication may be due to increased body weight of these patients. Therefore dietary consulting can be helpful in H. pylori infected patients for preventing of weight gain after eradication.
    Türk Biyokimya Dergisi / Turkish Journal of Biochemistry 01/2014; 39(2):196-200. DOI:10.5505/tjb.2014.71463 · 0.17 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: With the prevalence of cardio-metabolic disorders reaching pandemic proportions, the search for modifiable causative factors has intensified. One such potential factor is the vast microbial community inhabiting the human gastrointestinal tract, the gut microbiota. For the past decade evidence has accumulated showing the association of distinct changes in gut microbiota composition and function with obesity, type 2 diabetes and cardiovascular disease. Although causality in humans and the pathophysiological mechanisms involved have yet to be decisively established, several studies have demonstrated that the gut microbiota, as an environmental factor influencing the metabolic state of the host, is readily modifiable through a variety of interventions. In this review we provide an overview of the development of the gut microbiome and its compositional and functional changes in relation to cardio-metabolic disorders, and give an update on recent progress in how this could be exploited in microbiota-based therapeutics.
    Genome Medicine 12/2015; 7(1):33. DOI:10.1186/s13073-015-0157-z · 4.94 Impact Factor
  • Source
    05/2014; 3(3):44-57. DOI:10.7453/gahmj.2014.018