Article

C60-fullerene binds with the ATP binding domain of human DNA topoiosmerase II alpha.

Nanomaterial Toxicology Group, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M. G. Marg, Lucknow 226001, India.
Journal of Biomedical Nanotechnology (impact factor: 4.22). 02/2011; 7(1):177-8. pp.177-8
Source: PubMed

ABSTRACT C60-fullerene has promising biological applications, such as drug delivery, biosensors, diagnosis and theraupetics. Despite of these applications, several in vitro studies have also reported the DNA damaging potential of this nanomaterial. Though, very little is known about the mechanism involved behind the fullerene mediated DNA damage. Our study was aimed at identifying the binding site of fullerene in the ATP binding domain of human topoisomerase II alpha, a major enzyme involved in maintaining DNA topology. In silico studies of fullerene with the enzyme demonstrated that it can interact with the active site residues of this enzyme through hydrophobic, pi-stacking and van der Waals interactions and could inhibit the activity of this enzyme.

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Keywords

ATP binding domain
 
biosensors
 
DNA damage
 
DNA damaging potential
 
DNA topology
 
drug delivery
 
human topoisomerase II alpha
 
hydrophobic
 
pi-stacking
 
silico studies
 
theraupetics
 
van der Waals interactions
 
vitro studies