Article

Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization.

Centro de Referencia para Lactobacilos, CERELA-CONICET, Chacabuco 145 (4000), San Miguel de Tucumán, Tucumán, Argentina.
Biotechnology Letters (impact factor: 1.68). 04/2011; 33(8):1625-32. DOI:10.1007/s10529-011-0609-5
Source: PubMed

ABSTRACT PURPOSE OF WORK: To clone, express and characterize uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD) from Lactobacillus reuteri. Some strains of Lb. reuteri produce cobalamin (vitamin B(12)). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex metabolic pathway. We have cloned, expressed and characterized the gene in Lb. reuteri that codes for the S-adenosy L: -methionine uroprophyrinogen III methyltransferase/synthase (CobA/HemD), a key bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols.

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Keywords

-methionine uroprophyrinogen III methyltransferase/synthase
 
25 enzymes
 
biosynthesis
 
clone
 
cobalamin
 
Cobalamin biosynthesis
 
codes
 
complex metabolic pathway
 
key bifunctional enzyme
 
Lactobacillus reuteri
 
sequential action
 
uroporphyrinogen III synthase/methyltransferase gene