Fucoidan, a major component of brown seaweed, prohibits the growth of human cancer cell lines in vitro

Department of Pathology and Pediatric Surgery, Kurume University School of Medicine, Fukuoka 830-0011, Japan. .
Molecular Medicine Reports (Impact Factor: 1.55). 07/2008; 1(4):537-42. DOI: 10.3892/mmr.1.4.537
Source: PubMed


Fucoidan, the general term for sulfated polysaccharides, is reported to engage in various biological activities having anti-tumor, anti-coagulation and anti-viral effects. Though it has been investigated, the mechanism of its anti-tumor effects remains elusive. The current study examined the anti-tumor effects of fucoidan extracted from Okinawa mozuku on 15 human cancer cell lines (6 hepatocellular carcinomas, 1 cholangiocarcinoma, 1 gallbladder cancer, 2 ovarian cancers, 1 hepatoblastoma, 1 neuroblastoma and 3 renal cancers) using an MTT assay. Changes in apoptosis and the cell cycle were analyzed by flow cytometry. The results revealed that cell proliferation was suppressed in 13 cell lines in a time- and/or dose-dependent manner; this suppression was marked in the hepatocellular carcinoma, cholangiocarcinoma and gallbladder carcinoma cell lines. In contrast, proliferation of the neuroblastoma and 1 of the 2 ovarian carcinoma cell lines was not affected. The ratio of apoptotic cells significantly increased in 5 of the 6 hepatocellular carcinoma cell lines, and the ratio of G2/M cells increased in the 3 hepatocellular cell lines examined. These observations indicate that fucoidan is a potential anti-tumor agent for the treatment of bile duct cancers, such as hepatocellular carcinoma, cholangiocarcinoma and gall-bladder carcinoma.

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    • "Although fucoidan exerts various pharmacological activities, such as anti-inflammatory, antioxidant, and anticancer effects (Riou et al., 1996; Li et al., 2008; Fukahori et al., 2008; Saitoh et al., 2009; Fitton, 2011; Senni et al., 2011; Park et al., 2011b, 2013; Zhang et al., 2011, 2013; Wang et al., 2012; Lee et al., 2012; Liu et al., 2012; Hsu et al., 2013; Xue et al., 2013; Yang et al., 2013; Senthilkumar et al., 2013; Park et al., 2014; Banafa et al., 2013; Chen et al., 2014; Senthilkumar and Kim, 2014; Wang et al., 2014), the anti-cancer activity of fucoidan in human bladder cancer cells has rarely been reported. Therefore, we elucidated the ability of fucoidan to inhibit the growth of human bladder cancer EJ cells. "
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    ABSTRACT: Fucoidan, a sulfated polysaccharide found in marine algae and brown seaweeds, has been shown to inhibit the in vitro growth of human cancer cells. This study was conducted in cultured human bladder cancer EJ cells to elucidate the possible mechanisms by which fucoidan exerts its anti-proliferative activity, which until now has remained poorly understood. Fucoidan treatment of EJ cells resulted in dose-dependent inhibition of cell growth and induced apoptotic cell death. Flow cytometric analysis revealed that fucoidan led to G1 arrest in cell cycle progression. It was associated with down-regulation of cyclin D1, cyclin E, and cyclin-dependent-kinases (Cdks) in a concentration-dependent manner, without any change in Cdk inhibitors, such as p21 and p27. Furthermore, dephosphorylation of retinoblastoma protein (pRB) by this compound was associated with enhanced binding of pRB with the transcription factors E2F-1 and E2F-4. Overall, our results demonstrate that fucoidan possesses anticancer activity potential against bladder cancer cells by inhibiting pRB phosphorylation.
    Revista Brasileira de Farmacognosia 04/2015; 37(3). DOI:10.1016/j.bjp.2015.03.011 · 0.83 Impact Factor
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    • "These results confirm data obtained in previous studies. The sulfated polysaccharides from other species of brown algae were found to be nontoxic against JB6 Cl41 (epidermal mouse cells), Vero (African green monkey kidney), MCF-10A (human epithelial cells), MCF-7 (human breast cancer cells) and other cells [39,40,41]. Our results indicate that these polysaccharides are not cytotoxic towards DLD-1 human colon cancer cells at concentrations from 1 to 200 μg/mL. "
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    ABSTRACT: Three different fucoidan fractions were isolated and purified from the brown alga, Sargassum mcclurei. The SmF1 and SmF2 fucoidans are sulfated heteropolysaccharides that contain fucose, galactose, mannose, xylose and glucose. The SmF3 fucoidan is highly sulfated (35%) galactofucan, and the main chain of the polysaccharide contains a →3)-α-l-Fucp(2,4SO3-)-(1→3)-α-l-Fucp(2,4SO3-)-(1→ motif with 1,4-linked 3-sulfated α-l-Fucp inserts and 6-linked galactose on reducing end. Possible branching points include the 1,2,6- or 1,3,6-linked galactose and/or 1,3,4-linked fucose residues that could be glycosylated with terminal β-d-Galp residues or chains of alternating sulfated 1,3-linked α-l-Fucp and 1,4-linked β-d-Galp residues, which have been identified in galactofucans for the first time. Both α-l-Fucp and β-d-Galp residues are sulfated at C-2 and/or C-4 (and some C-6 of β-d-Galp) and potentially the C-3 of terminal β-d-Galp, 1,4-linked β-d-Galp and 1,4-linked α-l-Fucp residues. All fucoidans fractions were less cytotoxic and displayed colony formation inhibition in colon cancer DLD-1 cells. Therefore, these fucoidan fractions are potential antitumor agents.
    Marine Drugs 05/2013; 11(5):1456-76. DOI:10.3390/md11051456 · 2.85 Impact Factor
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    • "These results are in agreement with data from previous studies. The sulfated polysaccharides from other species of brown algae were found to be nontoxic against JB6 Cl41 (epidermal mouse cells), Vero (African green monkey kidney), MCF-10A (human epithelial cells), MCF-7 (human breast cancer cells) and other cells [26,27]. It has been reported that resveratrol was cytotoxic against different types of cancer; for example, the IC50 value of resveratrol against HCT 116 cells ranged from 35 to 100 μM [28,29]. "
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    ABSTRACT: Accumulating data clearly indicate that the induction of apoptosis by nontoxic natural compounds is a potent defense against the development and progression of many malignancies, including colon cancer. Resveratrol and the fucoidans have been shown to possess potent anti-tumor activity in vitro and in vivo. The aim of the present study was to examine whether the combination of a fucoidan from the brown alga Saccharina cichorioides Miyabe and resveratrol would be an effective preventive and/or therapeutic strategy against colon cancer. Based on NMR spectroscopy and MALDI-TOF analysis, the fucoidan isolated and purified from Saccharina cichorioides Miyabe was (1→3)-α-l-fucan with sulfate groups at C2 and C4 of the α-l-fucopyranose residues. The fucoidan enhanced the antiproliferative activity of resveratrol at nontoxic doses and facilitated resveratrol-induced apoptosis in the HCT 116 human colon cancer cell line. Apoptosis was realized by the activation of initiator caspase-9 and effector caspase-7 and -3, followed by the cleavage of PARP. Furthermore, significant inhibition of HCT 116 colony formation was associated with the sensitization of cells to resveratrol by the fucoidan. Taken together, these results demonstrate that the combination of the algal fucoidan with resveratrol may provide a potential therapy against human colon cancer.
    Marine Drugs 01/2013; 11(1):194-212. DOI:10.3390/md11010194 · 2.85 Impact Factor
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