Preparation and characterization of lyophilised egg PC liposomes incorporating curcumin and evaluation of its activity against colorectal cancer cell lines.
ABSTRACT Curcumin has been associated with the treatment of various diseases in traditional medicine, among them cancer. The major problems that prevent its approval as therapeutic agent are its low water solubility and its relatively low in vivo bioavailability. Liposomes are considered as effective drug carriers because of their ability to solubilize hydrophobic compounds and to alter their pharmacokinetic properties. The purpose of this study was the development of lyophilised liposomal curcumin fully characterized in terms of its physical properties [(zeta-potential, size, size distribution and Polydispercity index (PI)], and to evaluate its in vitro cytotoxic against colorectal cancer cell lines in a short-term and in a long-term (clonogenic) assay. Curcumin was incorporated in egg-phosphatidylcholine (EPC) liposomes at a drug to lipid molar ratio 1:14 achieving high incorporation efficiency close to 85%. The liposomal curcumin was lyophilized preserving thus its stability. The reconstitution of the formulation resulted in the original liposomal suspension. The release in FBS showed a plateau near 14% at 96 hours of incubation. The in vitro studies against colorectal cancer cell lines have shown that liposomes improve the activity of curcumin especially in the long-term assay and the liposomal formulation found to be more potent against HCT116 and HCT15, cell lines which express the MDR phenotype. EPC liposomal curcumin in a molar ratio of curcumin/EPC 1:14 has shown improved cytotoxic activity versus free curcumin against colorectal cancer cell lines. In vivo studies based on the recent findings are in progress in our laboratory.
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ABSTRACT: Cancer is a malignant disease with high mortality rates affecting millions. Although chemotherapeutic agents are employed largely in cancer management, they often result in toxicity and side effects and may lead to resistance. These draw backs of the conventional chemotherapeutic agents have lead to the urging need for development of safer, biocompatible, nontoxic compounds from natural sources and their application in cancer management. Products from natural sources are being exploited in cancer research worldwide due to its less toxicity. Of the several natural products tested, curcumin, well known for its chemopreventive, cytoprotective and immune suppressive properties holds great promise for cancer research. Curcumin has been reported to affect different signaling pathways either in a direct or indirect manner in a wide range of cancers. Despite the therapeutic effectiveness of curcumin, its application is largely restricted due to its poor absorption, lipophilic nature and low bioavailability. Thus newer and effective formulations for curcumin in cancer treatment are being continuously exploited. In this review we highlighted (i) recent developments of application of natural products in cancer research (ii) role of curcumin in different cancers (iii) curcumin formulations and their application in cancer research. The future scope of this review lies in the effective employment of curcumin and its formulations, in the eradication of cancer.Journal of Research in Biology. 05/2012; 3:251-272.
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ABSTRACT: Curcumin, a natural diphenolic compound derived from turmeric Curcuma longa, has proven to be a modulator of intracellular signaling pathways that control cancer cell growth, inflammation, invasion and apoptosis, revealing its anticancer potential. In this review, we focus on the design and development of nanoparticles, self-assemblies, nanogels, liposomes and complex fabrication for sustained and efficient curcumin delivery. We also discuss the anticancer applications and clinical benefits of nanocurcumin formulations. Only a few novel multifunctional and composite nanosystem strategies offer simultaneous therapy as well as imaging characteristics. We also summarize the challenges to developing curcumin delivery platforms and up-to-date solutions for improving curcumin bioavailability and anticancer potential for therapy.Drug discovery today 09/2011; 17(1-2):71-80. · 6.63 Impact Factor
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ABSTRACT: Curcumin (diferuloylmethane) is a yellow pigment present in the spice turmeric (Curcuma longa) that has been associated with antioxidant, anti-inflammatory, anticancer, antiviral, and antibacterial activities as indicated by over 6,000 citations. In addition, over one hundred clinical studies have been carried out with curcumin. One of the major problems with curcumin is perceived to be the bioavailability. How curcumin should be delivered in vivo, how bioavailable is it, how well curcumin is absorbed and how it is metabolized, is the focus of this review. Various formulations of curcumin that are currently available are also discussed.Cancer Research and Treatment 01/2014; 46(1):2-18. · 1.96 Impact Factor