Validated HPLC-UV method for the determination of berberine in raw herb Daruharidra (Berberis aristata DC), its extract, and in commercially marketed Ayurvedic dosage forms.
ABSTRACT A new, simple, sensitive, selective, and precise high-performance liquid chromatography (HPLC) method for analysis of berberine in crude plant material, herbal extract, and ayurvedic dosage forms was developed and validated. The stationary phase was inert sil C(18) column The mobile phase consisting of acetonitrile (HPLC Grade) and potassium dihydrogen phosphate buffer (pH 2.5) in a gradient flow was used.The column was equilibrated with the mobile phase (flow rate 1.0 ml/min); elution was monitored at 346 nm. The linear regression analysis data for the calibration plots showed good linear relationship, with r(2)= 0.9942 in the concentration range of 16380-30420 μg/ml with respect to the peak area. The method was validated for specificity, precision, recovery, and linearity according to the International Conference on Harmonization guidelines. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of berberine.
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ABSTRACT: The genus Berberis is well known for its diversity and pharmacological uses in traditional medicine system since ancient time. Exploring this medicinal plant with more prominence is the need of present day medicinal system. The present review highlighted the phytochemical and pharmacological studies reported from genus Berberis over the last two decades.Phytochemistry Reviews 12/2013; 11(4). DOI:10.1007/s11101-013-9272-x · 2.89 Impact Factor