Article

Acetate-glycerol cometabolism: cultivating Schizosaccharomyces pombe on a non-fermentable carbon source in a defined minimal medium.

AVT - Biochemical Engineering, RWTH Aachen University, Worringer Weg, D-52074 Aachen, Germany.
Journal of Bioscience and Bioengineering (impact factor: 1.79). 03/2011; 112(1):20-5. DOI:10.1016/j.jbiosc.2011.02.014 pp.20-5
Source: PubMed

ABSTRACT The growth of the fission yeast Schizosaccharomyces pombe on glucose and glycerol was monitored on-line in shake flasks and microtiter plates. The Edinburgh Minimal Medium 2 was improved by doubling its concentrations, improving its buffer and increasing its sulphur and iron concentrations additionally. By growing S. pombe on mixed carbon sources, it was shown that glycerol and glucose complement one another. Several tests were performed to establish the cultivation of S. pombe with non-fermentable glycerol as the main carbon source in minimal medium. Interestingly, a synergistic effect of glycerol and acetate was discovered which can significantly improve the growth of the fission yeast on glycerol. S. pombe showed optimal respiration activity, growth, and product formation by co-utilizing 20g/L glycerol and 2.5g/L sodium acetate.

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Keywords

2.5g/L sodium acetate
 
co-utilizing 20g/L glycerol
 
concentrations
 
Edinburgh Minimal Medium 2
 
fission yeast Schizosaccharomyces pombe
 
glycerol
 
iron concentrations additionally
 
main carbon source
 
microtiter plates
 
minimal medium
 
non-fermentable glycerol
 
on-line
 
optimal respiration activity
 
product formation
 
S. pombe
 
sulphur
 
synergistic effect