Effects of shear stress on the number and function of endothelial progenitor cells adhered to specific matrices

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, China.
Journal of applied biomaterials & biomechanics (JABB) (Impact Factor: 1.16). 03/2011; 9(3):193-8. DOI: 10.5301/JABB.2011.6475
Source: PubMed


The aim of this study was to screen specific adherent matrix for endothelial progenitor cells (EPCs), which can be used for antibody capturing stents.
In this study, the adhesion of EPCs on different matrices containing three different antibodies, VEGFR-2, CD34, CD133, was observed under shear stress in a flow chamber. Nitric oxide (NO) release, cell proliferation and the retention rate of EPCs, were measured separately.
The results demonstrated that shear stress within a certain range can promote proliferation and NO secretion of EPCs. Under the same shear stress, the EPCs showed stronger adhesion on matrix-containing CD133 antibody than on the other matrices.
CD133 antibody has the potential application for EPCs capture.

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