Dengue Infection Increases the Locomotor Activity of Aedes aegypti Females

Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
PLoS ONE (Impact Factor: 3.23). 03/2011; 6(3):e17690. DOI: 10.1371/journal.pone.0017690
Source: PubMed

ABSTRACT Aedes aegypti is the main vector of the virus causing Dengue fever, a disease that has increased dramatically in importance in recent decades, affecting many tropical and sub-tropical areas of the globe. It is known that viruses and other parasites can potentially alter vector behavior. We investigated whether infection with Dengue virus modifies the behavior of Aedes aegypti females with respect to their activity level.
We carried out intrathoracic Dengue 2 virus (DENV-2) infections in Aedes aegypti females and recorded their locomotor activity behavior. We observed an increase of up to ∼50% in the activity of infected mosquitoes compared to the uninfected controls.
Dengue infection alters mosquito locomotor activity behavior. We speculate that the higher levels of activity observed in infected Aedes aegypti females might involve the circadian clock. Further studies are needed to assess whether this behavioral change could have implications for the dynamics of Dengue virus transmission.

Download full-text


Available from: Marcia Castro, Sep 26, 2015
27 Reads
  • Source
    • "albopictus females when testing flight, sugar-feeding and host-seeking activities under laboratory conditions [18,28,29]. In addition, under laboratory conditions, it has been reported that parasite-vector interactions, such as dengue virus- Ae. aegypti infection, as well as genetic mutations which denote insecticide resistance by the vector do not change the pattern of activity of Ae. aegypti, but increase the locomotor activity of females [24,30]. Although it has been reported that physiological states, such as insemination and blood-feeding, affect the flight activity of Ae. aegypti females [18], it has not been evaluated up to now how drastic changes in these physiological states could affect the locomotor activity pattern of Ae. aegypti and Ae. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Dengue is an arbovirus disease transmitted by two Aedes mosquitoes: Ae. aegypti and Ae. albopictus. Virgin females of these two species generally show a bimodal and diurnal pattern of activity, with early morning and late afternoon peaks. Although some studies on the flight activity of virgin, inseminated and blood-fed Ae. aegypti females have been carried out under laboratory conditions, little is known about the effects of such physiological states on the locomotor activity of Ae. albopictus and Ae. aegypti females. The aim of this study was to analyze, under laboratory conditions, the effects of insemination and blood-feeding on the locomotor activity of Ae. albopictus and Ae. aegypti females under LD 12:12, at 25°C. Methods Both Ae. albopictus and Ae. aegypti females were obtained from established laboratory colonies. Control groups were represented by virgin/unfed Ae. albopictus and Ae. aegypti females. Experiments were conducted under laboratory conditions, using an activity monitor that registers individual activity every thirty minutes. Results Virgin/unfed Ae. albopictus and Ae. aegypti females showed a diurnal and bimodal pattern of locomotor activity, with peaks at early morning and late afternoon. Insemination and blood-feeding significantly decreased the locomotor activity of Ae. aegypti females, but inseminated/blood-fed Ae. aegypti and Ae. albopictus females showed a similar significant decrease on the locomotor activity compared to virgin/unfed females. Conclusions This study is the first demonstration of the effects of insemination and blood-feeding on the locomotor activity of Ae. albopictus and Ae. aegypti females under artificial conditions. Data suggest that Ae. albopictus and Ae. aegypti females respond in different ways to physiological status changes and such divergence between these two dengue vectors, associated with several ecological differences, could be related to the greater dengue vectorial capacity of Ae. aegypti in Americas in comparison to Ae. albopictus.
    Parasites & Vectors 07/2014; 7(1):304. DOI:10.1186/1756-3305-7-304 · 3.43 Impact Factor
  • Source
    • "Behavioural analysis -Circadian locomotor activity rhythms of Ae. aegypti were recorded automatically using a larger version of the Drosophila Activity Monitoring system (Trikinetics) as previously described (Gentile et al. 2009, Lima-Camara et al. 2011), in a Precision Scientific Incubator Mod. 818. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Mosquitoes are the culprits of some of the most important vector borne diseases. A species' potential as a vector is directly dependent on their pattern of behaviour, which is known to change according to the female's physiological status such as whether the female is virgin/mated and unfed/blood-fed. However, the molecular mechanism triggered by and/or responsible for such modulations in behaviour is poorly understood. Clock genes are known to be responsible for the control of circadian behaviour in several species. Here we investigate the impact mating and blood-feeding have upon the expression of these genes in the mosquito Aedes aegypti . We show that blood intake, but not insemination, is responsible for the down-regulation of clock genes. Using RNA interference, we observe a slight reduction in the evening activity peak in the fourth day after dstim injection. These data suggest that, as in Drosophila , clock gene expression, circadian behaviour and environmental light regimens are interconnected in Ae. aegypti .
    Memórias do Instituto Oswaldo Cruz 12/2013; 108 Suppl 1(Suppl 1):80-7. DOI:10.1590/0074-0276130471 · 1.59 Impact Factor
  • Source
    • "Locomotor activity analysis of mosquitoes can be useful for testing the effect of external or internal factors, such as dengue infection on adult female performance ( Lima-Camara et al. 2011 ). Moreover, previous studies have shown that mating has a significant effect on flight activity of many mosquito species, including Ae. aegypti ( Clements 1999 ). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Dengue is one of the world's most important mosquito-borne diseases and is usually transmitted by one of two vector species: Aedes aegypti or Aedes albopictus . These two diurnal mosquitoes are frequently found coexisting in similar habitats, enabling interactions between adults, such as cross-mating. The objective of this study was to assess cross-mating between Ae. aegypti females and Ae. albopictus males under artificial conditions and evaluate the locomotor activity of Ae. aegypti virgin females injected with male accessory gland (MAG) homogenates to infer the physiological and behavioural responses to interspecific mating. After seven days of exposure, 3.3-16% of Ae. aegypti females mated with Ae. albopictus males. Virgin Ae. aegypti females injected with conspecific and heterospecific MAGs showed a general decrease in locomotor activity compared to controls and were refractory to mating with conspecific males. The reduction in diurnal locomotor activity induced by injections of conspecific or heterospecific MAGs is consistent with regulation of female reproductive activities by male substances, which are capable of sterilising female Ae. aegypti through satyrisation by Ae. albopictus .
    Memórias do Instituto Oswaldo Cruz 12/2013; 108 Suppl 1(Suppl 1):18-25. DOI:10.1590/0074-0276130381 · 1.59 Impact Factor
Show more