Multiple facets of Arabidopsis seedling development require indole-3-butyric acid-derived auxin

Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA.
The Plant Cell (Impact Factor: 9.58). 03/2011; 23(3):984-99. DOI: 10.1105/tpc.111.083071
Source: PubMed

ABSTRACT Levels of auxin, which regulates both cell division and cell elongation in plant development, are controlled by synthesis, inactivation, transport, and the use of storage forms. However, the specific contributions of various inputs to the active auxin pool are not well understood. One auxin precursor is indole-3-butyric acid (IBA), which undergoes peroxisomal β-oxidation to release free indole-3-acetic acid (IAA). We identified ENOYL-COA HYDRATASE2 (ECH2) as an enzyme required for IBA response. Combining the ech2 mutant with previously identified iba response mutants resulted in enhanced IBA resistance, diverse auxin-related developmental defects, decreased auxin-responsive reporter activity in both untreated and auxin-treated seedlings, and decreased free IAA levels. The decreased auxin levels and responsiveness, along with the associated developmental defects, uncover previously unappreciated roles for IBA-derived IAA during seedling development, establish IBA as an important auxin precursor, and suggest that IBA-to-IAA conversion contributes to the positive feedback that maintains root auxin levels.

  • Source
    • "However, this is not supported by an ech2 ibr1 ibr3 ibr10 quadruple mutant, which has severe defects in the conversion of IBA to IAA in peroxisomes. The quadruple mutant exhibits smaller cotyledons, slower leaf development, and delayed flowering, but it has no gross morphological defects at maturity and its inflorescences are fertile and appear similar to those of wild-type plants (Strader et al., 2011). The pale green leaves of kat2 kat5 plants were reminiscent of the reduced chlorophyll observed in the pex5- 10 mutant that lacks a full-length PEX5 protein (Khan and Zolman, 2010). "
    [Show abstract] [Hide abstract]
    ABSTRACT: A specific function for peroxisomal β-oxidation in inflorescence development in Arabidopsis thaliana is suggested by the mutation of the ABNORMAL INFLORESCENCE MERISTEM 1 gene, which encodes one of two peroxisomal multifunctional proteins. Therefore, it should be possible to identify other β-oxidation mutants that recapitulate the aim1 phenotype. Three genes encode peroxisomal 3-ketoacyl-CoA thiolase (KAT) in Arabidopsis. KAT2 and KAT5 are present throughout angiosperms whereas KAT1 is a Brassicaceae-specific duplication of KAT2 expressed at low levels in Arabidopsis. KAT2 plays a dominant role in all known aspects of peroxisomal β-oxidation, including that of fatty acids, pro-auxins, jasmonate precursor oxophytodienoic acid, and trans-cinnamic acid. The functions of KAT1 and KAT5 are unknown. Since KAT5 is conserved throughout vascular plants and expressed strongly in flowers, kat2 kat5 double mutants were generated. These were slow growing, had abnormally branched inflorescences, and ectopic organ growth. They made viable pollen, but produced no seed indicating that infertility was due to defective gynaecium function. These phenotypes are strikingly similar to those of aim1. KAT5 in the Brassicaceae encodes both cytosolic and peroxisomal proteins and kat2 kat5 defects could be complemented by the re-introduction of peroxisomal (but not cytosolic) KAT5. It is concluded that peroxisomal KAT2 and KAT5 have partially redundant functions and operate downstream of AIM1 to provide β-oxidation functions essential for inflorescence development and fertility.
    Journal of Experimental Botany 10/2014; 65(22). DOI:10.1093/jxb/eru397 · 5.79 Impact Factor
  • Source
    • "Mutants with defects of peroxisomal biosynthesis or b-oxidation are resistant to externally applied IBA (Zolman et al., 2000, 2001a,b; Zolman & Bartel, 2004). Several mutants of peroxisomal enzymes appear to be solely linked to b-oxidation-like processing of IBA to IAA (Zolman et al., 2008; Strader et al., 2011). Moreover, mutants defective in b-oxidation are impaired in IBA to IAA conversion (Strader et al., 2010). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Controlled plant growth requires regulation through a variety of signaling molecules, including steroids, peptides, radicals of oxygen and nitrogen, as well as the 'classical' phytohormone groups. Auxin is critical for the control of plant growth and also orchestrates many developmental processes, such as the formation of new roots. It modulates root architecture both slowly, through actions at the transcriptional level and, more rapidly, by mechanisms targeting primarily plasma membrane sensory systems and intracellular signaling pathways. The latter reactions use several second messengers, including Ca(2+) , nitric oxide (NO) and reactive oxygen species (ROS). Here, we investigated the different roles of two auxins, the major auxin indole-3-acetic acid (IAA) and another endogenous auxin indole-3-butyric acid (IBA), in the lateral root formation process of Arabidopsis and maize. This was mainly analyzed by different types of fluorescence microscopy and inhibitors of NO production. This study revealed that peroxisomal IBA to IAA conversion is followed by peroxisomal NO, which is important for IBA-induced lateral root formation. We conclude that peroxisomal NO emerges as a new player in auxin-induced root organogenesis. In particular, the spatially and temporally coordinated release of NO and IAA from peroxisomes is behind the strong promotion of lateral root formation via IBA.
    New Phytologist 06/2013; 200(2). DOI:10.1111/nph.12377 · 7.67 Impact Factor
  • Source
    • "Seeds were surface sterilized , plated on medium containing 0 . 5% Suc ( PNS medium ) ( Zolman et al . , 2001 ; Strader et al . , 2011 ) , and then stratified for 3 d in the dark at 4°C . For root elongation assays , plates were subsequently incubated in constant light for 3 d , and then plants were transferred to fresh PNS plates lacking or containing the indicated amounts of hormones and incubated for 8 d in constant light . Plants were then removed and the primary r"
    [Show abstract] [Hide abstract]
    ABSTRACT: COMPARATIVE GENE IDENTIFICATION-58 (CGI-58) is a key regulator of lipid metabolism and signaling in mammals, but its underlying mechanisms are unclear. Disruption of CGI-58 in either mammals or plants results in a significant increase in triacylglycerol (TAG), suggesting that CGI-58 activity is evolutionarily conserved. However, plants lack proteins that are important for CGI-58 activity in mammals. Here, we demonstrate that CGI-58 functions by interacting with the PEROXISOMAL ABC-TRANSPORTER1 (PXA1), a protein that transports a variety of substrates into peroxisomes for their subsequent metabolism by β-oxidation, including fatty acids and lipophilic hormone precursors of the jasmonate and auxin biosynthetic pathways. We also show that mutant cgi-58 plants display changes in jasmonate biosynthesis, auxin signaling, and lipid metabolism consistent with reduced PXA1 activity in planta and that, based on the double mutant cgi-58 pxa1, PXA1 is epistatic to CGI-58 in all of these processes. However, CGI-58 was not required for the PXA1-dependent breakdown of TAG in germinated seeds. Collectively, the results reveal that CGI-58 positively regulates many aspects of PXA1 activity in plants and that these two proteins function to coregulate lipid metabolism and signaling, particularly in nonseed vegetative tissues. Similarities and differences of CGI-58 activity in plants versus animals are discussed.
    The Plant Cell 05/2013; 25(5). DOI:10.1105/tpc.113.111898 · 9.58 Impact Factor
Show more

Similar Publications