Evaluation of the Dengue NS1 Ag Strip® for detection of dengue virus antigen in Aedes aegypti (Diptera: Culicidae).
ABSTRACT Dengue fever is currently one of the most important mosquito-borne diseases that affect humans. With neither vaccines nor treatment available, prevention of the disease relies heavily on surveillance and control of mosquito vectors. In the present study, we have evaluated and showed the potential use of the Dengue NS1 Ag Strip(®) for the detection of dengue virus (DENV) in Aedes aegypti. Initial results showed that the sensitivity of the test kit in detecting DENV in wild-caught mosquitoes is comparable to that of real-time reverse transcriptase-polymerase chain reaction. The detection of naturally infected Ae. aegypti with the NS1 rapid test kit in our dengue cluster investigation further illustrates its potential use for surveillance of DENV in wild mosquito populations. The kit can easily be used in a simple field station, and minimal training is required. The results can be obtained in less than an hour. Employment of the kit in the field could help guide mosquito control operations in the prioritization of resources in controlling the transmission of DENV. In this study the potential of the kit for field surveillance of infected dengue vectors, which are epidemiologically important, has been demonstrated.
Full-textDOI: · Available from: Indra Vythilingam, Aug 09, 2015
- SourceAvailable from: Julian E. Garcia-Rejon
- "Surveillance of DENV in Ae. aegypti females is of potential interest as a means for early detection of dengue outbreaks because human DENV infections are commonly asymptomatic or " silent " (Gubler 1998, Kyle and Harris 2008), which decreases the effectiveness of dengue case surveillance to provide early warning of building outbreaks. Improved methods for detection of DENV RNA or antigen in mosquitoes (Bangs et al. 2007, Chao et al. 2007, Chen et al. 2010, Tan et al. 2011, Muller et al. 2012, Voge et al. 2013) provide new opportunities for mosquito-based DENV surveillance, and studies focusing entirely or in part on dengue patient premises have shown promise with regards to the potential for linking DENV infections in Ae. aegypti to human dengue cases in space and time (Pinheiro et al. 2005, Urdaneta et al. 2005, Garcõ´a- 886 JOURNAL OF MEDICAL ENTOMOLOGY Vol. 51, no. 4 Rejó n et al. 2008, Guedes et al. 2010, Yoon et al. 2012). "
Dataset: Eisen et al 2014 JME
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- "aegpypti male mosquito (15.2.3). The use of the NS1 antigen capture kit for the detection of DENV antigens from Ae. aegypti mosquitoes has recently been demonstrated (Tan et al. 2011). However, none of the other techniques that were available could confirm infection or verify the infecting serotype. "
ABSTRACT: In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes aegypti that were field-caught. Conventional reverse transcriptase-polymerase chain reaction (RT-PCR) and SimplexaTM Dengue real-time RT-PCR confirmed DENV-4 infection in all cases. Additionally, DENV-4 was confirmed in a female Ae. aegypti with 1.08 x 10³ copies/mL of virus, as determined by quantitative real-time RT-PCR. This is the first time the SimplexaTM Dengue real-time assay has been used for the classification of cases of infection and for entomological investigations. The use of these molecular techniques was shown to be important for the surveillance of dengue in humans and vectors.Memórias do Instituto Oswaldo Cruz 11/2012; 107(7):940-5. DOI:10.1590/S0074-02762012000700017 · 1.57 Impact Factor
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- "NS1 released in homogenates of infected mosquitoes prepared using a portable hand pestle was readily detected with a diagnostic NS1 rapid strip. NS1 detection in field-caught A. aegypti has recently been reported, providing proof-of-concept for field studies , but the method described in that report required specialized laboratory equipment and reagents unsuitable for onsite processing and detection (Tan et al., 2011). Using the approach outlined here, NS1 can be detected in pools of trapped mosquitoes with a crude extraction method that is rapid, requires no down-stream laboratory infrastructure or specialized staff, allows results to be obtained on-site in the field and for large numbers of mosquitoes to be processed in a single reaction. "
ABSTRACT: Dengue virus is the most significant human viral pathogen spread by the bite of an infected mosquito. With no vaccine or antiviral therapy currently available, disease prevention relies largely on surveillance and mosquito control. Preventing the onset of dengue outbreaks and effective vector management would be considerably enhanced through surveillance of dengue virus prevalence in natural mosquito populations. However, current approaches to the identification of virus in field-caught mosquitoes require relatively slow and labor intensive techniques such as virus isolation or RT-PCR involving specialized facilities and personnel. A rapid and portable method for detecting dengue virus-infected mosquitoes is described. Using a hand held battery operated homogenizer and a dengue diagnostic rapid strip the viral protein NS1 was detected as a marker of dengue virus infection. This method could be performed in less than 30 min in the field, requiring no downstream processing, and is able to detect a single infected mosquito in a pool of at least 50 uninfected mosquitoes. The method described in this study allows rapid, real-time monitoring of dengue virus presence in mosquito populations and could be a useful addition to effective monitoring and vector control responses.Journal of virological methods 07/2012; 183(1):90-3. DOI:10.1016/j.jviromet.2012.03.033 · 1.88 Impact Factor