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Evaluation of the Dengue NS1 Ag Strip® for detection of dengue virus antigen in Aedes aegypti (Diptera: Culicidae).

Environmental Health Institute, National Environmental Agency, Singapore, Singapore.
Vector borne and zoonotic diseases (Larchmont, N.Y.) (Impact Factor: 2.53). 03/2011; 11(6):789-92. DOI: 10.1089/vbz.2010.0028
Source: PubMed

ABSTRACT Dengue fever is currently one of the most important mosquito-borne diseases that affect humans. With neither vaccines nor treatment available, prevention of the disease relies heavily on surveillance and control of mosquito vectors. In the present study, we have evaluated and showed the potential use of the Dengue NS1 Ag Strip(®) for the detection of dengue virus (DENV) in Aedes aegypti. Initial results showed that the sensitivity of the test kit in detecting DENV in wild-caught mosquitoes is comparable to that of real-time reverse transcriptase-polymerase chain reaction. The detection of naturally infected Ae. aegypti with the NS1 rapid test kit in our dengue cluster investigation further illustrates its potential use for surveillance of DENV in wild mosquito populations. The kit can easily be used in a simple field station, and minimal training is required. The results can be obtained in less than an hour. Employment of the kit in the field could help guide mosquito control operations in the prioritization of resources in controlling the transmission of DENV. In this study the potential of the kit for field surveillance of infected dengue vectors, which are epidemiologically important, has been demonstrated.

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Available from: Indra Vythilingam, Aug 09, 2015
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    • "Surveillance of DENV in Ae. aegypti females is of potential interest as a means for early detection of dengue outbreaks because human DENV infections are commonly asymptomatic or " silent " (Gubler 1998, Kyle and Harris 2008), which decreases the effectiveness of dengue case surveillance to provide early warning of building outbreaks. Improved methods for detection of DENV RNA or antigen in mosquitoes (Bangs et al. 2007, Chao et al. 2007, Chen et al. 2010, Tan et al. 2011, Muller et al. 2012, Voge et al. 2013) provide new opportunities for mosquito-based DENV surveillance, and studies focusing entirely or in part on dengue patient premises have shown promise with regards to the potential for linking DENV infections in Ae. aegypti to human dengue cases in space and time (Pinheiro et al. 2005, Urdaneta et al. 2005, Garcõ´a- 886 JOURNAL OF MEDICAL ENTOMOLOGY Vol. 51, no. 4 Rejó n et al. 2008, Guedes et al. 2010, Yoon et al. 2012). "
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    • "aegpypti male mosquito (15.2.3). The use of the NS1 antigen capture kit for the detection of DENV antigens from Ae. aegypti mosquitoes has recently been demonstrated (Tan et al. 2011). However, none of the other techniques that were available could confirm infection or verify the infecting serotype. "
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    • "NS1 released in homogenates of infected mosquitoes prepared using a portable hand pestle was readily detected with a diagnostic NS1 rapid strip. NS1 detection in field-caught A. aegypti has recently been reported, providing proof-of-concept for field studies , but the method described in that report required specialized laboratory equipment and reagents unsuitable for onsite processing and detection (Tan et al., 2011). Using the approach outlined here, NS1 can be detected in pools of trapped mosquitoes with a crude extraction method that is rapid, requires no down-stream laboratory infrastructure or specialized staff, allows results to be obtained on-site in the field and for large numbers of mosquitoes to be processed in a single reaction. "
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